Version 1
: Received: 31 July 2023 / Approved: 1 August 2023 / Online: 2 August 2023 (07:32:13 CEST)
How to cite:
Noh, C.H.; Park, S.; Seong, H.; Lee, A.; Tsolmon, K.; Geum, D.H.; Hong, S.; Kim, T.M.; Choi, E.; Kim, Y. Wound-Healing Effects of an Exosome-Rich Conditioned Medium from Human Amniotic Membrane Stem Cells. Preprints2023, 2023080124. https://doi.org/10.20944/preprints202308.0124.v1
Noh, C.H.; Park, S.; Seong, H.; Lee, A.; Tsolmon, K.; Geum, D.H.; Hong, S.; Kim, T.M.; Choi, E.; Kim, Y. Wound-Healing Effects of an Exosome-Rich Conditioned Medium from Human Amniotic Membrane Stem Cells. Preprints 2023, 2023080124. https://doi.org/10.20944/preprints202308.0124.v1
Noh, C.H.; Park, S.; Seong, H.; Lee, A.; Tsolmon, K.; Geum, D.H.; Hong, S.; Kim, T.M.; Choi, E.; Kim, Y. Wound-Healing Effects of an Exosome-Rich Conditioned Medium from Human Amniotic Membrane Stem Cells. Preprints2023, 2023080124. https://doi.org/10.20944/preprints202308.0124.v1
APA Style
Noh, C.H., Park, S., Seong, H., Lee, A., Tsolmon, K., Geum, D.H., Hong, S., Kim, T.M., Choi, E., & Kim, Y. (2023). Wound-Healing Effects of an Exosome-Rich Conditioned Medium from Human Amniotic Membrane Stem Cells. Preprints. https://doi.org/10.20944/preprints202308.0124.v1
Chicago/Turabian Style
Noh, C.H., Ehn-Kyoung Choi and Yun-Bae Kim. 2023 "Wound-Healing Effects of an Exosome-Rich Conditioned Medium from Human Amniotic Membrane Stem Cells" Preprints. https://doi.org/10.20944/preprints202308.0124.v1
Abstract
Tissue regeneration is an essential requirement for wound healing and recovery of organs’ dysfunction. It has been demonstrated that wound healing can be facilitated by activating paracrine signaling mediated by exosomes secreted from stem cells, since exosomes deliver many functional molecules including growth factors (GFs) and neurotrophic factors (NFs) effective for tissue regeneration. In this study, an exosome-rich conditioned medium (ERCM) was collected from human amniotic membrane stem cells (AMSCs) by cultivating the cells under a low oxygen tension (2% O2 and 5% CO2). The contents of GFs and NFs including keratinocyte growth factor, epidermal growth factor, fibroblast growth factor 1, transforming growth factor-β, and vascular endothelial growth factor responsible for skin regeneration were much higher (10-30 folds) in the ERCM than in normal conditioned medium. In was found that CM-DiI-labeled exosomes readily entered keratinocytes and fibroblasts, and that ERCM not only facilitated the proliferation of keratinocytes in normal condition, but also protected against H2O2 cytotoxicity in a concentration-dependent manner. In cell-migration assay, the scratch wound in keratinocyte culture dish was rapidly closed by treatment with ERCM. Such wound-healing effects of ERCM were confirmed in a rat whole skin-excision model: i.e., the wound closure was significantly accelerated by topical application of ERCM solution (4 x 109 exosome particles/100 μL) at 4-day intervals. In the wounded skin, the deposition of collagens was enhanced by treatment with ERCM, which was supported by the increased production of collagen-1 and collagen-3. The results indicate that ERCM from AMSCs containing a large amount of GFs and NFs improve wound healing through tissue regeneration not only by facilitating keratinocyte proliferation for skin repair, but also activating fibroblasts for extracellular matrix production.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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