Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Seminal Oxidation–Reduction Potential Correlates with Not Only Seminal Oxidative But Also Nitrosative Stresses

Teppei Takeshima
* ORCID logo ,
Tomoki Saito
,
Kaoru Yoshida
ORCID logo ,
Yoko Akiba
,
Yosuke Takimoto
,
Yukari Ishiguro
,
Yasushi Yumura
ORCID logo
Version 1 : Received: 5 July 2023 / Approved: 6 July 2023 / Online: 6 July 2023 (08:37:32 CEST)

How to cite: Takeshima, T.; Saito, T.; Yoshida, K.; Akiba, Y.; Takimoto, Y.; Ishiguro, Y.; Yumura, Y. Seminal Oxidation–Reduction Potential Correlates with Not Only Seminal Oxidative But Also Nitrosative Stresses. Preprints 2023, 2023070393. https://doi.org/10.20944/preprints202307.0393.v1 Takeshima, T.; Saito, T.; Yoshida, K.; Akiba, Y.; Takimoto, Y.; Ishiguro, Y.; Yumura, Y. Seminal Oxidation–Reduction Potential Correlates with Not Only Seminal Oxidative But Also Nitrosative Stresses. Preprints 2023, 2023070393. https://doi.org/10.20944/preprints202307.0393.v1

Abstract

Reactive oxygen species and reactive nitrogen species in semen are both essential for fertilization process; however, they become harmful at excessive levels, causing oxidative stress and nitrosative stress, respectively. Recently, oxidation–reduction potential (ORP) has been used as a marker for measuring oxidative stress, and this study investigated the possibility of comprehensively evaluating nitrosative stress. The correlation between standardized ORP (sORP) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) and NOx per unit sperm was tested. Based on the previously reported cutoff value, samples were classified into oxidative stress-positive and negative groups. Thereafter, a receiver operating characteristic curve was drawn on 8-OHdG and NOx, and each optimal cutoff value was determined. Both standardized 8-OHdG and NOx significantly correlated with sORP. Although both correlated significantly with sORP in the oxidative stress group, no correlation was found in the nonoxidative stress group. The optimal standardized 8-OHdG level to determine oxidative stress status was 0.52 ng/106 spermatozoa, and the standardized NOx was 0.17 µM/106 spermatozoa/mL. The ORP is a simpler and quicker assay that can comprehensively assess oxidative and nitrosative stresses. Optimal cutoff values for both stresses were established in this study.

Keywords

oxidative stress; nitrosative stress; reactive oxygen species; reactive nitrogen species; oxidation–reduction potential

Subject

Medicine and Pharmacology, Urology and Nephrology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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