PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Unleashing the Ligno-Hemicellulolytic Enzyme Potential of Pyrenophora phaeocomes S-1: A Solid State Fermentation Approach for Efficient Delignification of Corn Stover
Version 1
: Received: 8 June 2023 / Approved: 9 June 2023 / Online: 9 June 2023 (10:28:39 CEST)
How to cite:
Rastogi, S.; Soni, R.; Sharma, A.; Soni, S. K. Unleashing the Ligno-Hemicellulolytic Enzyme Potential of Pyrenophora phaeocomes S-1: A Solid State Fermentation Approach for Efficient Delignification of Corn Stover. Preprints2023, 2023060699. https://doi.org/10.20944/preprints202306.0699.v1
Rastogi, S.; Soni, R.; Sharma, A.; Soni, S. K. Unleashing the Ligno-Hemicellulolytic Enzyme Potential of Pyrenophora phaeocomes S-1: A Solid State Fermentation Approach for Efficient Delignification of Corn Stover. Preprints 2023, 2023060699. https://doi.org/10.20944/preprints202306.0699.v1
Rastogi, S.; Soni, R.; Sharma, A.; Soni, S. K. Unleashing the Ligno-Hemicellulolytic Enzyme Potential of Pyrenophora phaeocomes S-1: A Solid State Fermentation Approach for Efficient Delignification of Corn Stover. Preprints2023, 2023060699. https://doi.org/10.20944/preprints202306.0699.v1
APA Style
Rastogi, S., Soni, R., Sharma, A., & Soni, S. K. (2023). Unleashing the Ligno-Hemicellulolytic Enzyme Potential of Pyrenophora phaeocomes S-1: A Solid State Fermentation Approach for Efficient Delignification of Corn Stover. Preprints. https://doi.org/10.20944/preprints202306.0699.v1
Chicago/Turabian Style
Rastogi, S., Apurav Sharma and Sanjeev Kumar Soni. 2023 "Unleashing the Ligno-Hemicellulolytic Enzyme Potential of Pyrenophora phaeocomes S-1: A Solid State Fermentation Approach for Efficient Delignification of Corn Stover" Preprints. https://doi.org/10.20944/preprints202306.0699.v1
Abstract
Biological pretreatment of lignocellulosic residues has the potential to serve as a sustainable, less energy-intensive alternative to harsh chemical treatments for enhancing cellulose accessibility, despite the requirement of a lengthy incubation period. The study characterized the simultaneous delignification of corn stover by the white-rot fungus Pyrenophora phaeocomes S-1, as well as the co-production of a ligno-hemicellulolytic enzyme cocktail consisting of laccase, xylanase, and mannanase. The maximum yields of all three components of the cocktail were achieved after just 4 days of incubation under solid-state conditions. Following a 40-day fermentation period, we achieved a cellulose recovery of 44.25 ± 1.72%, attributed to the activities of 133.88 U/gds of laccase, 14.93 U/gds of xylanase, and 1.34 U/gds of mannanase co-produced by the fungus in the medium. Subsequently, through the extraction of biologically treated biomass with 0.5N NaOH after 40 days, the cellulose recovery increased to 66.4 ± 1.39%. Enzymatic hydrolysis of the same, employing only 5 FPU/gds of in-house produced cellulases, resulted in the liberation of 397.84 mg/gds of total reducing sugars after 144h. The findings of this study encourage further optimization of biological pretreatment of lignocellulosic residues and enzymatic hydrolysis to enhance the yields of total reducing sugars for their valorization.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
