Article
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Preserved in Portico This version is not peer-reviewed
Survivals of Bacteriophage T4 in Quasi-Pure Ionic Solutions
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Version 1
: Received: 7 June 2023 / Approved: 7 June 2023 / Online: 7 June 2023 (07:05:54 CEST)
A peer-reviewed article of this Preprint also exists.
Hara, S.; Koike, I. Survival of Bacteriophage T4 in Quasi-Pure Ionic Solutions. Viruses 2023, 15, 1737, doi:10.3390/v15081737. Hara, S.; Koike, I. Survival of Bacteriophage T4 in Quasi-Pure Ionic Solutions. Viruses 2023, 15, 1737, doi:10.3390/v15081737.
Abstract
The preservative qualities of individual ionic compounds on the infectivity of T4 virions were elucidated. T4 virions were immersed in quasi-pure ionic solutions prior to the adsorption process, and the pfu values of these were measured following the conventional method. In neutral ionic solutions, the minimum and the optimum concentrations for preservative qualities were corresponded with the results obtained from the multi-ionic media/buffers. In acid and alkali solutions, phages show tolerances at a pH range of 5-11 in multi-ionic media/buffers. T4 virions show no tolerance in quasi-pure acid, neutral and weak alkaline conditions. The preservative quality of T4 virions was jumped up in over 10-1 mM OH- solution, equivalent to pH value over 10, which is corresponding to the pKa of the deprotonation of the DNA bases G and T. Infectivity was lost below 10-1 mM OH- and higher than 10 mM OH-. These results implicate the maintaining of infectivity of a virion may need the flexibility of the intra-capsid DNA by deprotonation.
Keywords
infectivity; neutral solution; acidic solution; alkaline solution; deprotonation
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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