Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Extensive Drug Resistance of Strong Biofilm-Producing Acinetobacter baumannii Strains Isolated from Infections and Colonization Hospitalized Patients in Southern Poland

Tomasz Kasperski
ORCID logo ,
Dorota Romaniszyn
,
Estera Jachowicz-Matczak
,
Monika Pomorska-Wesołowska
,
Jadwiga Wójkowska-Mach
,
Agnieszka Chmielarczyk
* ORCID logo
Version 1 : Received: 22 May 2023 / Approved: 23 May 2023 / Online: 23 May 2023 (10:33:26 CEST)

A peer-reviewed article of this Preprint also exists.

Kasperski, T.; Romaniszyn, D.; Jachowicz-Matczak, E.; Pomorska-Wesołowska, M.; Wójkowska-Mach, J.; Chmielarczyk, A. Extensive Drug Resistance of Strong Biofilm-Producing Acinetobacter baumannii Strains Isolated from Infections and Colonization Hospitalized Patients in Southern Poland. Pathogens 2023, 12, 975. Kasperski, T.; Romaniszyn, D.; Jachowicz-Matczak, E.; Pomorska-Wesołowska, M.; Wójkowska-Mach, J.; Chmielarczyk, A. Extensive Drug Resistance of Strong Biofilm-Producing Acinetobacter baumannii Strains Isolated from Infections and Colonization Hospitalized Patients in Southern Poland. Pathogens 2023, 12, 975.

Abstract

Acinetobacter baumannii (AB) is a bacterium that causes infections, particularly in immunocompromised patients. Treatment is challenging due to biofilm formation by AB strains, which hinders antibiotic effec-tiveness and promotes drug resistance. The aim of our study was to analyze the biofilm-producing capacity of AB isolates from various forms of infections in relation to biofilm-related genes and their drug resistance. We tested one hundred isolates for biofilm formation using the crystal violet microplate method. Drug re-sistance analyses were performed based on EUCAST and CLSI guidelines, and biofilm genes were detected using PCR. All tested strains were found to form biofilms, with 50% being ICU strains and 72% classified as strong biofilm-producers. Among these, 87% were extensively drug-resistant (XDR) and 2% were extra extensively drug-resistant (E-XDR). The most common gene set was bap, bfmS, csuE, and ompA, found in 57% of all isolates. Our research has shown that, regardless of the form of infection, biofilm-forming strains can be expected among AB isolates. The emergence of E-XDR and XDR strains among non-ICU infections highlights the necessity for the rational use of antibiotics to stop or limit the further acquisition of drug re-sistance by A. baumannii.

Keywords

Acinetobacter baumannii; biofilm; extensively drug resistance; biofilm associated genes

Subject

Medicine and Pharmacology, Epidemiology and Infectious Diseases

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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