Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Approaches to Enhancing Bioproduction using Lignocellulose Biomass: A Major Focus on Biofuel Production

Version 1 : Received: 9 October 2022 / Approved: 11 October 2022 / Online: 11 October 2022 (10:33:29 CEST)

How to cite: Ugwuoji, E.; Eruala, L.; Akpadolu, C.; Akpadolu, K. Approaches to Enhancing Bioproduction using Lignocellulose Biomass: A Major Focus on Biofuel Production. Preprints 2022, 2022100149. https://doi.org/10.20944/preprints202210.0149.v1 Ugwuoji, E.; Eruala, L.; Akpadolu, C.; Akpadolu, K. Approaches to Enhancing Bioproduction using Lignocellulose Biomass: A Major Focus on Biofuel Production. Preprints 2022, 2022100149. https://doi.org/10.20944/preprints202210.0149.v1

Abstract

The demand for an efficient utilization of abundant biomasses is growing for the production of biogas and valuable bioproducts. Lignocellulose biomass is a cheap and most abundant carbon source for the production of biofuels such as bioethanol, biobutanediol, and other bio-based chemicals. Due to its complex heterogeneity, its hydrolysis gives rise to a mixture of sugars, mainly glucose; a hexose and xylose; a pentose. Glucose is the most abundant carbohydrate monomer. Most microorganisms have evolved the ability to utilize it preferably due to carbon catabolite repression regulatory mechanism at the detriment of the pentoses. Some microbes even lack the ability to utilize them. This has led to the sequential use of these sugars and accompanying reduced productivity due to inadequate utilization of the pentoses. Also, this sequential utilization of the sugars takes time and makes the overall processes economically costly. Since lignocellulose hydrolysates comprise both hexoses and pentoses, the catabolism of these sugar mixtures to biofuels will require an efficient microbial strain capable of simultaneous utilization. The use of CCR negative mutants can achieve this. CCR negative mutants simultaneously utilize pentoses and hexoses, ensuring an improved fermentation efficacy and greater productivity, thus, making the overall bioprocess economically feasible. This article reviewed several approaches employed in creating these mutant microorganisms. A brief insight on carbon catabolite repression and phosphostransferase system were made. It also highlighted the biogas production processes, factors affecting anaerobic digestion, lignocellulosic biomass structure, challenges with their use and solutions to overcoming the challenges.

Keywords

Biogas; anaerobic digestion; gene mutation; bioengineering; lignocellulose biomass

Subject

Biology and Life Sciences, Biology and Biotechnology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.