PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Design of Experimental Approach Biosensor for the Development and Validation of UV Spectrophotometry Method for Determination of Thiamine in Biological Samples and Tablet Formulation
Version 1
: Received: 13 June 2021 / Approved: 14 June 2021 / Online: 14 June 2021 (16:04:21 CEST)
How to cite:
Sharma, H.; Sapkota, H.P.; Aryal, S.; Dangi, N.B.; BATIHA, G.E.; Koirala, N. Design of Experimental Approach Biosensor for the Development and Validation of UV Spectrophotometry Method for Determination of Thiamine in Biological Samples and Tablet Formulation. Preprints2021, 2021060379. https://doi.org/10.20944/preprints202106.0379.v1
Sharma, H.; Sapkota, H.P.; Aryal, S.; Dangi, N.B.; BATIHA, G.E.; Koirala, N. Design of Experimental Approach Biosensor for the Development and Validation of UV Spectrophotometry Method for Determination of Thiamine in Biological Samples and Tablet Formulation. Preprints 2021, 2021060379. https://doi.org/10.20944/preprints202106.0379.v1
Sharma, H.; Sapkota, H.P.; Aryal, S.; Dangi, N.B.; BATIHA, G.E.; Koirala, N. Design of Experimental Approach Biosensor for the Development and Validation of UV Spectrophotometry Method for Determination of Thiamine in Biological Samples and Tablet Formulation. Preprints2021, 2021060379. https://doi.org/10.20944/preprints202106.0379.v1
APA Style
Sharma, H., Sapkota, H.P., Aryal, S., Dangi, N.B., BATIHA, G.E., & Koirala, N. (2021). Design of Experimental Approach Biosensor for the Development and Validation of UV Spectrophotometry Method for Determination of Thiamine in Biological Samples and Tablet Formulation. Preprints. https://doi.org/10.20944/preprints202106.0379.v1
Chicago/Turabian Style
Sharma, H., Gaber El-Saber BATIHA and Niranjan Koirala. 2021 "Design of Experimental Approach Biosensor for the Development and Validation of UV Spectrophotometry Method for Determination of Thiamine in Biological Samples and Tablet Formulation" Preprints. https://doi.org/10.20944/preprints202106.0379.v1
Abstract
UV-Visible spectroscopy method has been developed for the analysis of Thiamine in biological and pharmaceutical product, based on a chromogenic derivatizing reaction using PDAB (P- dimethyl amino benzaldehyde) reagent. A Central Composite Design (CCD) design with response surface methodology was executed for optimization of experimental conditions of drug with a smaller number of experimental trials. Three independent factors, the concentration of PDAB, the volume of PDAB reagent, and volume of HCl, were used to construct a mathematical model and study the effects of these independent factors on responses as absorbance. The colored complex exhibits a Red shift with absorption maximum λmax at 425 nm, which was selected as the analytical wavelength. The drug seems to be linear, which was established via the regression analysis from 5-30 μgmL-1, with an R2 value of 0.998.The % RSD for intraday and interday precision was < 2%, with good recovery ranging from 95.02 to 101.43% with biological and pharmaceutical samples. LOD and LOQ of the developed method were found to be 1.51 μg mL-1 and 4.57 μg mL-1.This method can be used in routine analysis of pharmaceutical products containing aromatic primary amines along with an estimation of biological samples like urine, blood, sweat, faeces.
Keywords
thiamine; PDAB reagent; UV Spectrophotometry; Design of experiment; pharmaceutical samples
Subject
Medicine and Pharmacology, Immunology and Allergy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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