Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Genome-Wide Role of HSF1 in Transcriptional Regulation of Desiccation Tolerance in the Anhydrobiotic Cell Line, Pv11

Shoko Tokumoto
,
Yugo Miyata
ORCID logo ,
Ruslan Deviatiiarov
,
Takahiro G. Yamada
ORCID logo ,
Yusuke Hiki
,
Olga Kozlova
,
Yuki Yoshida
,
Richard Cornette
ORCID logo ,
Akira Funahashi
ORCID logo ,
Elena Shagimardanova
ORCID logo ,
Oleg Gusev
ORCID logo ,
Takahiro Kikawada
* ORCID logo
Version 1 : Received: 16 April 2021 / Approved: 19 April 2021 / Online: 19 April 2021 (12:17:34 CEST)

A peer-reviewed article of this Preprint also exists.

Tokumoto, S.; Miyata, Y.; Deviatiiarov, R.; Yamada, T.G.; Hiki, Y.; Kozlova, O.; Yoshida, Y.; Cornette, R.; Funahashi, A.; Shagimardanova, E.; Gusev, O.; Kikawada, T. Genome-Wide Role of HSF1 in Transcriptional Regulation of Desiccation Tolerance in the Anhydrobiotic Cell Line, Pv11. Int. J. Mol. Sci. 2021, 22, 5798. Tokumoto, S.; Miyata, Y.; Deviatiiarov, R.; Yamada, T.G.; Hiki, Y.; Kozlova, O.; Yoshida, Y.; Cornette, R.; Funahashi, A.; Shagimardanova, E.; Gusev, O.; Kikawada, T. Genome-Wide Role of HSF1 in Transcriptional Regulation of Desiccation Tolerance in the Anhydrobiotic Cell Line, Pv11. Int. J. Mol. Sci. 2021, 22, 5798.

Abstract

Pv11, an insect cell line established from the midge Polypedilum vanderplanki, is capable of ametabolic desiccation tolerance, so-called anhydrobiosis. We previously discovered that heat shock factor 1 (HSF1) contributes to the acquisition of desiccation tolerance by Pv11 cells, but the mechanistic details have yet to be elucidated. Here, by analyzing the gene expression profiles of newly established HSF1-knockout and -rescue cell lines, we show that HSF1 has a genome-wide effect on gene regulation in Pv11. HSF1-knockout cells exhibit a reduced desiccation survival rate, but this is completely restored in HSF1-rescue cells. By comparing mRNA profiles of the two cell lines, we reveal that HSF1 induces anhydrobiosis-related genes, especially genes encoding late embryogenesis abundant proteins and thioredoxins, but represses a group of genes involved in basal cellular processes, thus promoting an ametabolic state in the cell. In addition, HSF1 binding motifs are enriched in the promoters of anhydrobiosis-related genes and we demonstrate binding of HSF1 to these promoters by ChIP-qPCR. Thus, HSF1 directly regulates the transcription of anhydrobiosis-related genes and consequently plays a pivotal role in the induction of anhydrobiotic ability in Pv11 cells.

Keywords

CRISPR/Cas9; knockout; rescue; desiccation tolerance; anhydrobiosis; Polypedilum vanderplanki; HSF1; insect cell; Pv11

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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