PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and Human Cells
Version 1
: Received: 11 December 2019 / Approved: 12 December 2019 / Online: 12 December 2019 (05:24:29 CET)
How to cite:
Reigada, I.; Pérez-Tanoira, R.; Patel, J.; Savijoki, K.; Yli-Kauhaluoma, J.; Kinnari, T. J.; Fallarero, A. Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and Human Cells. Preprints2019, 2019120164. https://doi.org/10.20944/preprints201912.0164.v1
Reigada, I.; Pérez-Tanoira, R.; Patel, J.; Savijoki, K.; Yli-Kauhaluoma, J.; Kinnari, T. J.; Fallarero, A. Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and Human Cells. Preprints 2019, 2019120164. https://doi.org/10.20944/preprints201912.0164.v1
Reigada, I.; Pérez-Tanoira, R.; Patel, J.; Savijoki, K.; Yli-Kauhaluoma, J.; Kinnari, T. J.; Fallarero, A. Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and Human Cells. Preprints2019, 2019120164. https://doi.org/10.20944/preprints201912.0164.v1
APA Style
Reigada, I., Pérez-Tanoira, R., Patel, J., Savijoki, K., Yli-Kauhaluoma, J., Kinnari, T. J., & Fallarero, A. (2019). Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by <em>Staphylococcus aureus </em>and Human Cells. Preprints. https://doi.org/10.20944/preprints201912.0164.v1
Chicago/Turabian Style
Reigada, I., Teemu J. Kinnari and Adyary Fallarero. 2019 "Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by <em>Staphylococcus aureus </em>and Human Cells" Preprints. https://doi.org/10.20944/preprints201912.0164.v1
Abstract
Biofilm-mediated infection is a major cause of bone prosthesis failure. The lack of molecules able to act in biofilms has driven research aimed at identifying new anti-biofilm agents via chemical screens. However, to be able to accommodate a large number of compounds, the testing conditions of these screenings end up being typically far from the clinical scenario. In this study, we assess the potential applicability of three anti-biofilm compounds (based on natural compounds) as part of implanted medical devices by testing them on in vitro systems that more faithfully resemble the clinical scenario. To that end, we used a competition model based on the co-culture of SaOS-2 mammalian cells and Staphylococcus aureus (collection and clinical strains) on a titanium surface. Additionally, we studied whether these derivatives of natural compounds enhance the previously proven protective effect of pre-incubating the titanium surface with SaOS-2 cells. Out of the three tested leads, one showed the highest potential, and can be regarded as a promising agent for incorporation into bone implants. This study emphasizes and demonstrates the importance of using meaningful experimental models, where potential antimicrobials ought to be tested for protection of biomaterials in translational applications.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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