Version 1
: Received: 29 November 2019 / Approved: 30 November 2019 / Online: 30 November 2019 (12:38:21 CET)
How to cite:
Johansson, M.; Ulfenborg, B.; X Andersson, C.; Hagvall, S.; Jeppsson, A.; Sartipy, P.; Synnergren, J. Cardiac Hypertrophy in a Dish: A Human Stem Cell Based Model. Preprints2019, 2019110395. https://doi.org/10.20944/preprints201911.0395.v1
Johansson, M.; Ulfenborg, B.; X Andersson, C.; Hagvall, S.; Jeppsson, A.; Sartipy, P.; Synnergren, J. Cardiac Hypertrophy in a Dish: A Human Stem Cell Based Model. Preprints 2019, 2019110395. https://doi.org/10.20944/preprints201911.0395.v1
Johansson, M.; Ulfenborg, B.; X Andersson, C.; Hagvall, S.; Jeppsson, A.; Sartipy, P.; Synnergren, J. Cardiac Hypertrophy in a Dish: A Human Stem Cell Based Model. Preprints2019, 2019110395. https://doi.org/10.20944/preprints201911.0395.v1
APA Style
Johansson, M., Ulfenborg, B., X Andersson, C., Hagvall, S., Jeppsson, A., Sartipy, P., & Synnergren, J. (2019). Cardiac Hypertrophy in a Dish: A Human Stem Cell Based Model. Preprints. https://doi.org/10.20944/preprints201911.0395.v1
Chicago/Turabian Style
Johansson, M., Peter Sartipy and Jane Synnergren. 2019 "Cardiac Hypertrophy in a Dish: A Human Stem Cell Based Model" Preprints. https://doi.org/10.20944/preprints201911.0395.v1
Abstract
Cardiac hypertrophy is an important and independent risk factor for the development of heart failure. To better understand the mechanisms and regulatory pathways involved in cardiac hypertrophy, there is a need for improved in vitro models. In this study, we investigated how hypertrophic stimulation affected human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CMs). The cells were stimulated with endothelin-1 (ET-1) for 8, 24, 48, 72, or 96h. Parameters including cell size, ANP-, proBNP-, and lactate concentration were analyzed. Moreover, transcriptional profiling using RNA-sequencing was performed to identify differentially expressed genes following ET-1 stimulation. The results show that the CMs increase in size by approximately 13% when exposed to ET-1 in parallel to increases in ANP and proBNP protein and mRNA levels. Furthermore, the lactate concentration in the media was significantly increased indicating that the CMs consume more glucose, a hallmark of cardiac hypertrophy. Using RNA-seq, a hypertrophic gene expression pattern was also observed in the stimulated CMs. Taken together, these results show that hiPSC-derived CMs stimulated with ET-1 display a hypertrophic response. The results from this study also provide new molecular insights about the underlying mechanisms of cardiac hypertrophy and may help accelerate the development of new drugs against this condition.
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
