Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Frozen Microemulsions for MAPLE Immobilization of Lipase

Valeria Califano
,
Francesco Bloisi
* ORCID logo ,
Giuseppe Perretta
,
Antonio Aronne
,
Giovanni Ausanio
,
Aniello Costantini
,
Luciano Vicari
Version 1 : Received: 21 September 2017 / Approved: 21 September 2017 / Online: 21 September 2017 (17:10:01 CEST)

A peer-reviewed article of this Preprint also exists.

Califano, V.; Bloisi, F.; Perretta, G.; Aronne, A.; Ausanio, G.; Costantini, A.; Vicari, L. Frozen Microemulsions for MAPLE Immobilization of Lipase. Molecules 2017, 22, 2153. Califano, V.; Bloisi, F.; Perretta, G.; Aronne, A.; Ausanio, G.; Costantini, A.; Vicari, L. Frozen Microemulsions for MAPLE Immobilization of Lipase. Molecules 2017, 22, 2153.

Abstract

MAPLE (matrix assisted pulsed laser evaporation) depositions of Candida Rugosa lipase were carried out from ice matrices whose composition is optimized in order to minimize conformational damage of the protein, which strongly influences its catalytic activity. To induce lid opening and to protect lipase during the MAPLE process, pentane and m-DOPA amino acid were added to the liquid matrix giving a target formed by a frozen water-lipase-pentane microemulsion. FTIR and AFM were used to investigate the structure of MAPLE deposited lipase films. The ability of MAPLE films to promote transesterification was determined by thin layer chromatography. It was shown that m-DOPA has influence on the aggregation but not on the unfolding of lipase induced by MAPLE, while the microemulsion formed by the addition of pentane to the target composition is effective in protecting lipase during the MAPLE process. MAPLE deposited lipases showed a modified specificity.

Keywords

MAPLE; Matrix Assisted Pulsed Laser Evaporation; microemulsion; lipase; thin film

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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