Preprint Article Version 1 NOT YET PEER-REVIEWED

Batch Fermentation Options for High Titer Bioethanol Production from a SPORL Pretreated Douglas-fir Forest Residue without Detoxification

Mingyan Yang 1,2,3 , Hairui Ji 2,4 , J.Y. ZHU 2,*
  1. School Environment Science Engineering, Chang’an University, Xi’an 710064, Chin
  2. USDA Forest Service, Forest Products Laboratory, Madison, WI 53726, USA
  3. Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region, Ministry of Education, Chang’an University, Xi’an 710064, China
  4. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Version 1 : Received: 6 August 2016 / Approved: 8 August 2016 / Online: 8 August 2016 (10:39:56 CEST)

A peer-reviewed article of this Preprint also exists.

Yang, M.; Ji, H.; Zhu, J. Batch Fermentation Options for High Titer Bioethanol Production from a SPORL Pretreated Douglas-Fir Forest Residue without Detoxification. Fermentation 2016, 2, 16. Yang, M.; Ji, H.; Zhu, J. Batch Fermentation Options for High Titer Bioethanol Production from a SPORL Pretreated Douglas-Fir Forest Residue without Detoxification. Fermentation 2016, 2, 16.

Journal reference: Fermentation 2016, 2, 16
DOI: 10.3390/fermentation2030016

Abstract

This study evaluated batch fermentation modes, namely, separate hydrolysis and fermentation (SHF), Quasi-simultaneous saccharification and fermentation (Q-SSF), and simultaneous saccharification and fermentation (SSF), and fermentation conditions, i.e., enzyme and yeast loadings, nutrient supplementation and sterilization, on high titer bioethanol production from SPORL-pretreated Douglas-fir forest residue without detoxification. The result indicated Q-SSF and SSF were obviously superior to SHF operation in terms of ethanol yield. The enzyme loading showed a strong positive correlation between enzyme loading and the ethanol yield. The nutrient supplementation and sterility was not necessary for ethanol production from SPORL-pretreated Douglas-fir. The yeast loading showed no significant influence on the ethanol yield for typical SSF conditions. The terminal ethanol titer of 43.2 g/L, or 75.1% theoretical based on glucose, mannose, and xylose theoretical was achieved when SSF was conducted at the condition of following: whole slurry solids loading of 15%, enzyme loading of 20 FPU/g glucan, 1.8 g/kg (wet) yeast loading, without nutrition supplementation and sterilization, at 38°C, on shake flask at 150 rpm for 96h. It is believed that with mechanical mixing, enzyme loading can be substantially reduced with affect ethanol yield by using a long fermentation time.

Subject Areas

forest residue; pretreatment; liquefaction; enzymatic hydrolysis/saccharification; fermentation; high titer bioethanol; detoxification

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