Preprint Article Version 1 NOT YET PEER-REVIEWED

Batch Fermentation Options for High Titer Bioethanol Production from a SPORL Pretreated Douglas-fir Forest Residue without Detoxification

Mingyan Yang 1,2,3 , Hairui Ji 2,4 , J.Y. ZHU 2,*
1
School Environment Science Engineering, Chang’an University, Xi’an 710064, Chin
2
USDA Forest Service, Forest Products Laboratory, Madison, WI 53726, USA
3
Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region, Ministry of Education, Chang’an University, Xi’an 710064, China
4
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Version 1 : Received: 6 August 2016 / Approved: 8 August 2016 / Online: 8 August 2016 (10:39:56 CEST)

A peer-reviewed article of this Preprint also exists.

Yang, M.; Ji, H.; Zhu, J. Batch Fermentation Options for High Titer Bioethanol Production from a SPORL Pretreated Douglas-Fir Forest Residue without Detoxification. Fermentation 2016, 2, 16. Yang, M.; Ji, H.; Zhu, J. Batch Fermentation Options for High Titer Bioethanol Production from a SPORL Pretreated Douglas-Fir Forest Residue without Detoxification. Fermentation 2016, 2, 16.

Journal reference: Fermentation 2016, 2, 16
DOI: 10.3390/fermentation2030016

Abstract

This study evaluated batch fermentation modes, namely, separate hydrolysis and fermentation (SHF), Quasi-simultaneous saccharification and fermentation (Q-SSF), and simultaneous saccharification and fermentation (SSF), and fermentation conditions, i.e., enzyme and yeast loadings, nutrient supplementation and sterilization, on high titer bioethanol production from SPORL-pretreated Douglas-fir forest residue without detoxification. The result indicated Q-SSF and SSF were obviously superior to SHF operation in terms of ethanol yield. The enzyme loading showed a strong positive correlation between enzyme loading and the ethanol yield. The nutrient supplementation and sterility was not necessary for ethanol production from SPORL-pretreated Douglas-fir. The yeast loading showed no significant influence on the ethanol yield for typical SSF conditions. The terminal ethanol titer of 43.2 g/L, or 75.1% theoretical based on glucose, mannose, and xylose theoretical was achieved when SSF was conducted at the condition of following: whole slurry solids loading of 15%, enzyme loading of 20 FPU/g glucan, 1.8 g/kg (wet) yeast loading, without nutrition supplementation and sterilization, at 38°C, on shake flask at 150 rpm for 96h. It is believed that with mechanical mixing, enzyme loading can be substantially reduced with affect ethanol yield by using a long fermentation time.

Subject Areas

forest residue; pretreatment; liquefaction; enzymatic hydrolysis/saccharification; fermentation; high titer bioethanol; detoxification

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