Psychrophilic anaerobic digestion emerges as an appealing integrated solution for the management of agricultural waste, particularly for farmers in regions where the average temperature does not exceed 26°C, as seen in coffee cultivation. Therefore, this study seeks to assess the biomethane potential of thermochemical treated coffee husk through psychrophilic anaerobic digestion (C3-20°C-w/pretreatment). To examine its viability, outcomes were compared with reactors operating at both mesophilic (C1-35°C) and psychrophilic (C2-20°C) conditions, albeit without the use of pretreated coffee husk. The C3-20°C-w/pretreatment test demonstrated a 36.89% increase (150.47 mL CH4/g VS; 161.04 mL CH4/g COD), while the C1-35°C test exhibited a 24.03% increase (124.99 mL CH4/g VS; 133.77 mL CH4/g COD), both in comparison to the C2-20°C test (94.96 mL CH4/g VS; 101.63 mL CH4/g COD). Notably, the C3-20°C-w/pretreatment trial yielded superior outcomes, accompanied by an associated energy output of 4262.2 KWh/year, sufficient to meet the annual energy demands of 588 residences. This marks an increase of 100 and 217 residences compared to mesophilic and psychrophilic AD of CH without pretreatment, respectively.

Medicinal plants are being tested as options to prevent infectious diseases in many countries. Baccharis crispa Spreng. (Asteraceae), is a native medicinal species widely used throughout South America. Our objective was to evaluate the antiviral activity of four wild populations of B. crispa from the province of Córdoba (Argentina) and determine if there is variability between them. The cytotoxicity of the extracts (chlorofom, ethanol and aquose) was evaluated by the neutral red uptake method. Using the plaque-forming unit reduction assay, virucidal and antiviral activity against Herpes Simplex type 1 and Chikungunya viruses were tested. Phytochemical screening of each extract was also performed. Puesto Pedernera (PP) population showed the best antiviral and virucidal effect, standing out the ethanolic extract as the least toxic and with the best inhibitory activity against both viral models. Villa del Parque (VP) population was inactive, regardless of the extract and the virus tested. The results revealed that extracts bioactivities vary significantly depending on the geographic area of collection, with PP and VP populations showing the most significant variability, not only in activity but also in chemical composition. These findings support the potential use of extracts as a source of therapeutic agents against different viruses.