Version 1
: Received: 25 February 2024 / Approved: 26 February 2024 / Online: 26 February 2024 (19:49:05 CET)
How to cite:
Lawrence, P. Numerical Investigation of Micro-gravity Field in a Cylindrical Cell Culture Flask Mounted in a Random Positioning Machine. Preprints2024, 2024021491. https://doi.org/10.20944/preprints202402.1491.v1
Lawrence, P. Numerical Investigation of Micro-gravity Field in a Cylindrical Cell Culture Flask Mounted in a Random Positioning Machine. Preprints 2024, 2024021491. https://doi.org/10.20944/preprints202402.1491.v1
Lawrence, P. Numerical Investigation of Micro-gravity Field in a Cylindrical Cell Culture Flask Mounted in a Random Positioning Machine. Preprints2024, 2024021491. https://doi.org/10.20944/preprints202402.1491.v1
APA Style
Lawrence, P. (2024). Numerical Investigation of Micro-gravity Field in a Cylindrical Cell Culture Flask Mounted in a Random Positioning Machine. Preprints. https://doi.org/10.20944/preprints202402.1491.v1
Chicago/Turabian Style
Lawrence, P. 2024 "Numerical Investigation of Micro-gravity Field in a Cylindrical Cell Culture Flask Mounted in a Random Positioning Machine" Preprints. https://doi.org/10.20944/preprints202402.1491.v1
Abstract
To establish simulated micro-gravity conditions, biological organisms are placed on a simulated microgravity system, which primarily rotate in two or three dimensions and rely on slow sedimentation with latency to detect gravity. Fluid dynamics arise as a consequence of rotation of the chambers with in which the cells are filled. To minimize the effects of centrifugal forces, it is necessary to position the samples as close to the center of rotation as possible. Strong shear forces and velocities within the vessel can have an adverse impact on cells, making this a critical topic in cell biology. During experimental investigation, this specific phenomenon is not apparent, but numerical modelling readily illustrates and is not widely analyzed in literature's. Furthermore, bulk flask are frequently employed on random positioning machines (RPM), not merely because they demand a substantial quantity of reagents. The flask presented in the study can be easily developed and fitted in conventional cell culture plates containing medium and reagents similar to those used in ground experiments, thus allowing it to remain close to the center of rotation. In the present study, a series of fluid dynamics simulations are performed to examine the impact of shear forces within the microvessel presented under varying conditions using a foreign medium condition, and these results are compared with existing vessels by observing the effects of shear forces and maximum velocity zones.
Keywords
tissue engineering; random positioning machines (RPMs); computational fluid dynamics(CFD); porous medium
Subject
Engineering, Mechanical Engineering
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
