PreprintArticleVersion 2Preserved in Portico This version is not peer-reviewed
Development and Validation of an Analytical Method for the Simultaneous Detection of Sulfonamides, Trimethoprim and Dapsone in Honey by Liquid Chromatography-Tandem Mass Spectrometry
Version 1
: Received: 22 December 2023 / Approved: 25 December 2023 / Online: 25 December 2023 (05:43:19 CET)
Version 2
: Received: 23 March 2024 / Approved: 25 March 2024 / Online: 25 March 2024 (11:17:28 CET)
How to cite:
Vesa, G.V.; Luca, A.; Gheiti-Heghedus, A.I.; Colobatiu, L.M.; Cioca, A.; Poenaru, M.; Mihaiu, M. Development and Validation of an Analytical Method for the Simultaneous Detection of Sulfonamides, Trimethoprim and Dapsone in Honey by Liquid Chromatography-Tandem Mass Spectrometry. Preprints2023, 2023121801. https://doi.org/10.20944/preprints202312.1801.v2
Vesa, G.V.; Luca, A.; Gheiti-Heghedus, A.I.; Colobatiu, L.M.; Cioca, A.; Poenaru, M.; Mihaiu, M. Development and Validation of an Analytical Method for the Simultaneous Detection of Sulfonamides, Trimethoprim and Dapsone in Honey by Liquid Chromatography-Tandem Mass Spectrometry. Preprints 2023, 2023121801. https://doi.org/10.20944/preprints202312.1801.v2
Vesa, G.V.; Luca, A.; Gheiti-Heghedus, A.I.; Colobatiu, L.M.; Cioca, A.; Poenaru, M.; Mihaiu, M. Development and Validation of an Analytical Method for the Simultaneous Detection of Sulfonamides, Trimethoprim and Dapsone in Honey by Liquid Chromatography-Tandem Mass Spectrometry. Preprints2023, 2023121801. https://doi.org/10.20944/preprints202312.1801.v2
APA Style
Vesa, G.V., Luca, A., Gheiti-Heghedus, A.I., Colobatiu, L.M., Cioca, A., Poenaru, M., & Mihaiu, M. (2024). Development and Validation of an Analytical Method for the Simultaneous Detection of Sulfonamides, Trimethoprim and Dapsone in Honey by Liquid Chromatography-Tandem Mass Spectrometry. Preprints. https://doi.org/10.20944/preprints202312.1801.v2
Chicago/Turabian Style
Vesa, G.V., Maria-Magdalena Poenaru and Marian Mihaiu. 2024 "Development and Validation of an Analytical Method for the Simultaneous Detection of Sulfonamides, Trimethoprim and Dapsone in Honey by Liquid Chromatography-Tandem Mass Spectrometry" Preprints. https://doi.org/10.20944/preprints202312.1801.v2
Abstract
The presence of antibiotic residues in honey has recently become a growing public health concern due to the overuse of these products in beekeeping and their transfer to the food industry. The aim of this study was to develop and validate a simple, selective and sensitive method for the simultaneous identification and quantification of residues of twenty-two sulfonamides, trimethoprim and dapsone in honey using a rapid and reliable liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. The developed method showed good linearity, specificity, precision (repeatability and intra-laboratory reproducibility) and recovery. The decision limit (CCalpha) ranged between 2.16-22.12 µg/kg for CCα, while the detection capability (CCbeta) was between 2.16- 2.67-27.36 µg/kg. The method proved to be rapid, reliable and effective for the determination of sulfonamides, trimethoprim and dapsone from honey samples. The average recoveries were estimated to be between 70 and 106%. The relative standard deviations (RSDs) were between 6-18%. In practice, the method is essential to check the honey sold on the market and ensure its safety before it reaches the consumer.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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