Toxicity Evaluation and Control Release of Curcumin-Loaded Amphiphilic Poly-n-vinylpyrrolidone Nanoparticles for the Treatment of Malignant Tumors: In Vitro and In Vivo Models

Anna L. Luss; Dmitry V. Bagrov; Anne V. Yagolovich; Ekaterina V. Kukovyakina; Irina I. Khan; Vadim S. Pokrovsky; Maria V. Shestovskaya; Marine E. Gasparian; Dmitry A. Dolgikh; Andrey N. Kuskov

doi:10.20944/preprints202312.1140.v1

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preprints.org > chemistry and materials science > biomaterials > doi: 10.20944/preprints202312.1140.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Toxicity Evaluation and Control Release of Curcumin-Loaded Amphiphilic Poly-n-vinylpyrrolidone Nanoparticles for the Treatment of Malignant Tumors: In Vitro and In Vivo Models

Anna L. Luss

^* ,

Dmitry V. Bagrov

Anne V. Yagolovich

Ekaterina V. Kukovyakina

Irina I. Khan

Vadim S. Pokrovsky

Maria V. Shestovskaya

Marine E. Gasparian

Dmitry A. Dolgikh

Andrey N. Kuskov

Version 1 : Received: 14 December 2023 / Approved: 15 December 2023 / Online: 15 December 2023 (08:25:26 CET)

A peer-reviewed article of this Preprint also exists.

Luss, A.L.; Bagrov, D.V.; Yagolovich, A.V.; Kukovyakina, E.V.; Khan, I.I.; Pokrovsky, V.S.; Shestovskaya, M.V.; Gasparian, M.E.; Dolgikh, D.A.; Kuskov, A.N. Toxicity Evaluation and Controlled-Release of Curcumin-Loaded Amphiphilic Poly-N-vinylpyrrolidone Nanoparticles: In Vitro and In Vivo Models. Pharmaceutics 2024, 16, 8. Luss, A.L.; Bagrov, D.V.; Yagolovich, A.V.; Kukovyakina, E.V.; Khan, I.I.; Pokrovsky, V.S.; Shestovskaya, M.V.; Gasparian, M.E.; Dolgikh, D.A.; Kuskov, A.N. Toxicity Evaluation and Controlled-Release of Curcumin-Loaded Amphiphilic Poly-N-vinylpyrrolidone Nanoparticles: In Vitro and In Vivo Models. Pharmaceutics 2024, 16, 8.

Abstract

Curcumin attracts a huge attention because of its biological properties: antiproliferative, antioxidant, anti-inflammatory, immunomodulatory and so on. However, its usage has been limited by poor water solubility and low bioavailability. Herein, to solve these problems we developed curcumin-loaded nanoparticles based on end-capped amphiphilic poly(N-vinylpyrrolidone). Nanoparticles were obtained using the suspension method and were characterized by dynamic and electrophoretic light scattering, transmission electron microscopy and atomic force microscopy. The average particle size was 200 nm, and ζ-potential was -4 mV. Curcumin release studies showed that nanoparticles are stable in water solution. In vitro release study showed prolonged action in gastric, intestinal and colonic fluids, consistently and in PBS. In vitro studies on epidermoid carcinoma and human embryonic kidney cells showed that the cells absorbed more curcumin in nanoparticles compared to pure curcumin. Nanoparticles are safe for healthy cells and show high cytotoxicity for glioblastoma cells in cytotoxicity studies in vitro. Median lethal dose was determined in acute toxicity assay on Zebrafish and was 23 μM. Overall, the curcumin-loaded nanoparticles seem promising for cancer treatment.

Keywords

curcumin; poly-n-vinylpyrrolidone; nanoparticles; micelles; glioblastoma; toxicity

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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