Dibdiakova, J.; Matic, J.; Wubshet, S.G.; Uhl, W.; Manamperuma, L.D.; Rusten, B.; Vik, E.A. Membrane Separation of Chicken Byproduct Hydrolysate for Up-Concentration of Bioactive Peptides. Membranes2024, 14, 28.
Dibdiakova, J.; Matic, J.; Wubshet, S.G.; Uhl, W.; Manamperuma, L.D.; Rusten, B.; Vik, E.A. Membrane Separation of Chicken Byproduct Hydrolysate for Up-Concentration of Bioactive Peptides. Membranes 2024, 14, 28.
Dibdiakova, J.; Matic, J.; Wubshet, S.G.; Uhl, W.; Manamperuma, L.D.; Rusten, B.; Vik, E.A. Membrane Separation of Chicken Byproduct Hydrolysate for Up-Concentration of Bioactive Peptides. Membranes2024, 14, 28.
Dibdiakova, J.; Matic, J.; Wubshet, S.G.; Uhl, W.; Manamperuma, L.D.; Rusten, B.; Vik, E.A. Membrane Separation of Chicken Byproduct Hydrolysate for Up-Concentration of Bioactive Peptides. Membranes 2024, 14, 28.
Abstract
Membrane processes, such as microfiltration, ultrafiltration, and nanofiltration are increasingly used for various applications in both upstream and downstream processing. Membrane-based processes play a critical role in the field of separation/purification of biotechnological products, including protein production/purification. The possibility of using membranes to separate peptides from a chicken byproduct hydrolysate and the effect of the performed downstream processing on the DPP-IV inhibitory activity of MDCR (mechanical deboning chicken residue) has been investigated. The chicken byproduct hydrolysate was prepared by enzymatic hydrolysis followed by MF, UF, NF, and RO separation. The LC-OCD analysis confirmed that NF and RO would retain the bioactive peptides in the concentrate in comparison to MF and UF. Bioactivity was correlated with molecular weight distribution profiles and average molecular weights. Permeates after ultrafiltration showed an IC50 value of 0.75 mg/mL, comparable to other potent DPP-IV inhibitors derived from various food sources, and significantly more potent compared to the microfiltration sample, which showed an IC50 value of 1.04 mg/mL. The average molecular weight of the permeates calculated from the SEC chromatograms was 883 g/mol for UF and 1437 g/mol for MF. Of the four membranes studied, the UF membrane showed the best separation properties with respect to maximizing the yield and up-concentration of the bioactive peptides. Overall, UF was demonstrated to be a feasible technology for the removal of the undesired high molecular weight substances and up-concentration of small molecular weight bioactive peptides from chicken byproduct hydrolysate.
Keywords
bioactive peptides; enzymatic protein hydrolysis; membrane filtration; purification; LC-OCD.
Subject
Chemistry and Materials Science, Biomaterials
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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