Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effect of Electrical Stimulation on PC12 Cells Cultured in Different Hydrogels: Basis for the Development of Biomaterials in Peripheral Nerve Tissue Engineering

Yusser Olguín
* ORCID logo ,
Mónica V. Selva
,
Diego Benavente
,
Nicole Orellana
,
Iván Montenegro
ORCID logo ,
Alejandro Madrid-Villegas
ORCID logo ,
Diego Jaramillo-Pinto
ORCID logo ,
María Carolina Otero
ORCID logo ,
Tomas P. Corrales
,
Cristian A. Acevedo
Version 1 : Received: 20 October 2023 / Approved: 20 October 2023 / Online: 23 October 2023 (11:14:08 CEST)

A peer-reviewed article of this Preprint also exists.

Olguín, Y.; Selva, M.; Benavente, D.; Orellana, N.; Montenegro, I.; Madrid, A.; Jaramillo-Pinto, D.; Otero, M.C.; Corrales, T.P.; Acevedo, C.A. Effect of Electrical Stimulation on PC12 Cells Cultured in Different Hydrogels: Basis for the Development of Biomaterials in Peripheral Nerve Tissue Engineering. Pharmaceutics 2023, 15, 2760. Olguín, Y.; Selva, M.; Benavente, D.; Orellana, N.; Montenegro, I.; Madrid, A.; Jaramillo-Pinto, D.; Otero, M.C.; Corrales, T.P.; Acevedo, C.A. Effect of Electrical Stimulation on PC12 Cells Cultured in Different Hydrogels: Basis for the Development of Biomaterials in Peripheral Nerve Tissue Engineering. Pharmaceutics 2023, 15, 2760.

Abstract

Extensive damage to peripheral nerves is a health problem with few therapeutic alternatives. In this context, the development of tissue engineering seeks to obtain materials that can help recreate environments conducive to cellular development and functional repair of peripheral nerves. Different hydrogels have been studied and presented as alternatives for future treatments to emulate the morphological characteristics of nerves. Along with this, other research proposes the need to incorporate electrical stimuli into treatments as agents that promote cell growth and differentiation; however, no precedent correlates the simultaneous effects of the types of hydrogel and electrical stimuli. In this research, the neural differentiation of PC12 cells we are evaluated, relating the effect of collagen, alginate, GelMA, and PEGDA hydrogels with electrical stimulation modulated in four different ways. Our results show significant correlations for different culture conditions, allowing us to develop new experimental schemes for new materials in peripheral nerve engineering.

Keywords

peripheral nerve tissue engineering; electrical stimulation; hydrogels; PC12 cells

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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