PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Fabrication and Characterization of Tissue-Engineered Carboxymethyl Cellulose Scaffolds Containing Spider Silk-Derived Fibroin Protein for Osteogenic Differentiation
Version 1
: Received: 26 June 2023 / Approved: 26 June 2023 / Online: 26 June 2023 (10:47:20 CEST)
How to cite:
Lee, W. J.; Cho, K.; Kim, A. Y.; Kim, G. W. Fabrication and Characterization of Tissue-Engineered Carboxymethyl Cellulose Scaffolds Containing Spider Silk-Derived Fibroin Protein for Osteogenic Differentiation. Preprints2023, 2023061795. https://doi.org/10.20944/preprints202306.1795.v1
Lee, W. J.; Cho, K.; Kim, A. Y.; Kim, G. W. Fabrication and Characterization of Tissue-Engineered Carboxymethyl Cellulose Scaffolds Containing Spider Silk-Derived Fibroin Protein for Osteogenic Differentiation. Preprints 2023, 2023061795. https://doi.org/10.20944/preprints202306.1795.v1
Lee, W. J.; Cho, K.; Kim, A. Y.; Kim, G. W. Fabrication and Characterization of Tissue-Engineered Carboxymethyl Cellulose Scaffolds Containing Spider Silk-Derived Fibroin Protein for Osteogenic Differentiation. Preprints2023, 2023061795. https://doi.org/10.20944/preprints202306.1795.v1
APA Style
Lee, W. J., Cho, K., Kim, A. Y., & Kim, G. W. (2023). Fabrication and Characterization of Tissue-Engineered Carboxymethyl Cellulose Scaffolds Containing Spider Silk-Derived Fibroin Protein for Osteogenic Differentiation. Preprints. https://doi.org/10.20944/preprints202306.1795.v1
Chicago/Turabian Style
Lee, W. J., Aaron Youngjae Kim and Gyung Whan Kim. 2023 "Fabrication and Characterization of Tissue-Engineered Carboxymethyl Cellulose Scaffolds Containing Spider Silk-Derived Fibroin Protein for Osteogenic Differentiation" Preprints. https://doi.org/10.20944/preprints202306.1795.v1
Abstract
This study aimed to investigate the characteristics of composite scaffolds in combination of fibroin and carboxymethyl cellulose (CMC) in bone tissue engineering. Fibroin is a useful biomaterial and is a major component of silk yarns composed of fibrous proteins. Improving for binding efficiency in bone formation after implanted scaffold, CMC was added to fibroin. CMC could improve injectable characters in bone substitutes by adding in scaffold material, fibroin. The porous shapes, porosity, surface wettability, water absorption, and thermal properties of the CMC added fibroin scaffold were better than fibroin only scaffold. For tissue engineering of bone marrow mesenchymal stem cells (BMSCs), BMSCs isolated from mice were seeded onto the scaffold, and the cell proliferation rate was measured. alkaline phosphatase activity in BMSCs was higher in the scaffold containing CMC than that in the scaffold containing fibroin alone. The expression levels of osteocyte marker genes and proteins were increased in the CMC scaffold. The biocompatibility and hydrophilicity of CMC scaffolds play important roles in the growth and proliferation of osteocytes. Furthermore, the CMC scaffold design proposed in this study could play an important role in facilitating cartilage, ossification, and nerve differentiation of BMSCs.
Keywords
fibroin, scaffold; carboxymethyl cellulose (CMC); osteocyte; regenerative medicine
Subject
Engineering, Bioengineering
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
