Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Introducing a High Throughput Nanozymatic Method for Green Nanozyme-Mediated Degradation of Organic Dyes in Real Water Media

Version 1 : Received: 10 June 2023 / Approved: 12 June 2023 / Online: 12 June 2023 (07:38:56 CEST)

How to cite: Hormozi Jangi, S.R. Introducing a High Throughput Nanozymatic Method for Green Nanozyme-Mediated Degradation of Organic Dyes in Real Water Media. Preprints 2023, 2023060791. https://doi.org/10.20944/preprints202306.0791.v1 Hormozi Jangi, S.R. Introducing a High Throughput Nanozymatic Method for Green Nanozyme-Mediated Degradation of Organic Dyes in Real Water Media. Preprints 2023, 2023060791. https://doi.org/10.20944/preprints202306.0791.v1

Abstract

In this work, a high throughput nanozymatic method was developed for green nanozyme-mediated biodegradation of organic dyes in real water media. The nanozymes were synthesized and characterized for their size and morphology by TEM and DLS analysis. The nanozymatic properties of the as-prepared nanomaterials were evaluated by standard enzyme activity assay, revealing high peroxidase-like performances for the as-prepared nanozymes. Therefore, for exploring more precise, the kinetics behavior of the as-prepared nanozymes was evaluated by the standard Lineweaver-Burk method, revealing a Vmax as high as 0.263 µM sec-1 and a Km as low as 0.03 mM, revealing high catalytic efficiency and affinity of the as-prepared nanozymes. Hence, the as-prepared nanozymes were utilized for organic dye degradation in real water media using methylene blue as a model organic dye. The effective factors on the dye degradation including pH, ionic strength, degradation time, nanozyme amount, etc. were optimized by the one-factor-at-a-time optimization method. At optimal experimental conditions, the as-prepared nanozymes can degrade about 99.0% of the organic dye within a degradation time as low as 7 min. The developed method was finally employed for the nanozyme-mediated degradation of methylene blue from real water media such as pool, mineral, river, and tap water samples. The results of this study revealed an excellent biodegradation yield of over 94.3%-99.0% for the different real samples, proving the suitability of the developed method for dye degradation in real water media.

Keywords

nanozyme-mediated dye degradation; organic dye; nanozyme; methylene blue; real water media

Subject

Chemistry and Materials Science, Analytical Chemistry

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