PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Electrostatic Assembly of Anti-Listeria Bacteriophages on a Self-Assembled Monolayer of Aminoundecanethiol: Film Morphology and Charge Transfer Studies
Fernandes, P.M.V.; Maciel, C.; Teixeira, P.; Pereira, C.M.; Campiña, J.M. Electrostatic Assembly of Anti-Listeria Bacteriophages on a Self-Assembled Monolayer of Aminoundecanethiol: Film Morphology, Charge Transfer Studies, and Infectivity Assays. Surfaces2023, 6, 114-132.
Fernandes, P.M.V.; Maciel, C.; Teixeira, P.; Pereira, C.M.; Campiña, J.M. Electrostatic Assembly of Anti-Listeria Bacteriophages on a Self-Assembled Monolayer of Aminoundecanethiol: Film Morphology, Charge Transfer Studies, and Infectivity Assays. Surfaces 2023, 6, 114-132.
Fernandes, P.M.V.; Maciel, C.; Teixeira, P.; Pereira, C.M.; Campiña, J.M. Electrostatic Assembly of Anti-Listeria Bacteriophages on a Self-Assembled Monolayer of Aminoundecanethiol: Film Morphology, Charge Transfer Studies, and Infectivity Assays. Surfaces2023, 6, 114-132.
Fernandes, P.M.V.; Maciel, C.; Teixeira, P.; Pereira, C.M.; Campiña, J.M. Electrostatic Assembly of Anti-Listeria Bacteriophages on a Self-Assembled Monolayer of Aminoundecanethiol: Film Morphology, Charge Transfer Studies, and Infectivity Assays. Surfaces 2023, 6, 114-132.
Abstract
The integration of bacteriophages, a particular class of viruses that specifically infect bacteria and archaea, in biosensors for the monitoring of pathogens in foods and beverages is highly desirable. To this end, an increasing focus has been set on the exploration of covalent and physical methods for the immobilization of phages on solid surfaces. This work investigates the electrostatic assembly of tailed phages, specifically anti-Listeria monocytogenes P100 phages, on an ultrathin self-assembled monolayer (SAM) of 11-amino-1-undecanethiol (AUT). The cationic properties of AUT may allow for the electrostatic capture of P100 in a capsid-down fashion, thus, exposing the specific receptor-binding proteins on their tails to the corresponding pathogens in analytical samples. The physical properties of immobilized phages have been studied using AFM, SEM, and electrochemical techniques, providing insight into the orientation of the phages and revealing that the pH plays a remarkable role in the morphology and charge transfer behavior of the adsorbed films. Overall, this research portrays SAMs of amino-akylthiols as a valid platform for the oriented immobilization of bacteriophages on surfaces for electroanalytical purposes.
Chemistry and Materials Science, Surfaces, Coatings and Films
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
