Preprint Article Version 1 NOT YET PEER-REVIEWED

IQGAP1 in Podosome /Invadosome is Involved in the Progression of Glioblastoma Multiforme Depending on the Tumor Status

  1. Laboratorio de Biología del Desarrollo. UD de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias (CIBICAN), Universidad de La Laguna, La Laguna, Av. Astrofísico Sánchez s/n. 38206 La Laguna. Tenerife. Spain
  2. CNR – National Research Council, Institute of Endocrinology and Experimental Oncology (IEOS), Via Sergio Pansini, 5 – 80131, Naples, Italy
  3. Service of Medical Oncology, University Hospital Nuestra Señora de Candelaria, Santa Cruz de Tenerife 38010, Canary Islands, Spain
  4. Medical Oncology, Hospiten® Hospitals, Tenerife, Spain
  5. Service of Pathology, University Hospital Nuestra Señora de Candelaria, Santa Cruz de Tenerife 38010, Canary Islands, Spain
  6. Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom
  7. Center of Excellence in Genomic Medicine Research (CEGMR), King Fahd Medical Research Center (KFMRC), Faculty of Applied Medical Sciences, King AbdulAziz University, Jeddah, 21589, Kingdom of Saudi Arabia
Version 1 : Received: 17 November 2016 / Approved: 24 November 2016 / Online: 24 November 2016 (18:02:20 CET)

How to cite: Rotoli, D.; Pérez-Rodríguez, N.; Morales, M.; Maeso, M.; Ávila, J.; Mobasheri, A.; Martín-Vasallo, P. IQGAP1 in Podosome /Invadosome is Involved in the Progression of Glioblastoma Multiforme Depending on the Tumor Status. Preprints 2016, 2016110126 (doi: 10.20944/preprints201611.0126.v1). Rotoli, D.; Pérez-Rodríguez, N.; Morales, M.; Maeso, M.; Ávila, J.; Mobasheri, A.; Martín-Vasallo, P. IQGAP1 in Podosome /Invadosome is Involved in the Progression of Glioblastoma Multiforme Depending on the Tumor Status. Preprints 2016, 2016110126 (doi: 10.20944/preprints201611.0126.v1).

Abstract

Glioblastoma multiforme (GBM) is the most frequent and aggressive primary brain tumor. GBM is formed by a very heterogeneous astrocyte population, neurons, neovascularization and infiltrating myeloid cells (microglia and monocyte derived macrophages). The IQGAP1 scaffold protein interacts with components of the cytoskeleton, cell adhesion molecules, and several signaling molecules to regulate cell morphology and motility, cell cycle and other cellular functions. IQGAP1 overexpression and delocalization has been observed in several tumors, suggesting a role for this protein in cell proliferation, transformation and invasion. IQGAP1 has been identified as a marker of amplifying cancer cells in GBMs. To determine the involvement of IQGAP1 in the onco-biology of GBM, we performed immunohistochemical confocal microscopical analysis of the IQGAP1 protein in human GBM tissue samples using cell type-specific markers. IQGAP1 immunostaining and subcellular localization was heterogeneous; the protein was located in the plasma membrane and, at variable levels, in nucleus and/or cytosol). Moreover, IQGAP1 positive staining was found in podosome/invadopodia-like structures. IQGAP1+ staining was observed in neurons (Map2+ cells), in cancer stem cells (CSC; nestin+) and in several macrophages (CD31+ or Iba1+). Our results indicate that the IQGAP1 protein is involved in normal cell physiology and also in oncologic processes.

Subject Areas

IQGAP1; GBM; scaffold protein; podosome/invadosome

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