Version 1
: Received: 5 November 2016 / Approved: 7 November 2016 / Online: 7 November 2016 (07:54:10 CET)
How to cite:
Penniston, J.; Gueguim Kana, E.B. Impact of Culture pH Regulation on Biohydrogen Production using Suspended and Immobilized Microbial Cells. Preprints2016, 2016110042. https://doi.org/10.20944/preprints201611.0042.v1
Penniston, J.; Gueguim Kana, E.B. Impact of Culture pH Regulation on Biohydrogen Production using Suspended and Immobilized Microbial Cells. Preprints 2016, 2016110042. https://doi.org/10.20944/preprints201611.0042.v1
Penniston, J.; Gueguim Kana, E.B. Impact of Culture pH Regulation on Biohydrogen Production using Suspended and Immobilized Microbial Cells. Preprints2016, 2016110042. https://doi.org/10.20944/preprints201611.0042.v1
APA Style
Penniston, J., & Gueguim Kana, E.B. (2016). Impact of Culture pH Regulation on Biohydrogen Production using Suspended and Immobilized Microbial Cells. Preprints. https://doi.org/10.20944/preprints201611.0042.v1
Chicago/Turabian Style
Penniston, J. and Evariste Bosco Gueguim Kana. 2016 "Impact of Culture pH Regulation on Biohydrogen Production using Suspended and Immobilized Microbial Cells" Preprints. https://doi.org/10.20944/preprints201611.0042.v1
Abstract
The effect of pH regulation on biohydrogen production was studied using suspended and immobilized mixed cultures. Four sets of experiments were conducted using suspended cells under regulated pH (Sus_R) and non-regulated pH conditions (Sus_N) as well as alginate-immobilized cells under pH regulated (Imm_R) and non-pH regulated conditions (Imm_N). Sus_R showed a peak hydrogen fraction of 44% and complete glucose degradation, compared to Sus_N with a peak hydrogen fraction of 36% and a glucose degradation of 37%. Imm_R experiments showed a peak biohydrogen fraction of 35%, while the peak hydrogen fraction observed with Imm_N was 22%. The highest hydrogen fraction was observed using suspended cells under regulated pH conditions. A 100% glucose degradation was observed in both pH regulated and non-regulated processes using immobilized cells. The rate of pH change was slower for immobilized cells compared to suspended cells suggesting a better buffering capacity under non pH regulated conditions. The study showed that biohydrogen production with suspended cells in a non-regulated pH environment resulted in early termination of the process and lower productivity.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
