Department of Molecular Pathology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan
Division of Pathology, Biological Safety Research Center, National Institute of Health Sciences, Ministry of Health, Labour and Welfare, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan
: Received: 17 August 2016 / Approved: 17 August 2016 / Online: 17 August 2016 (11:57:56 CEST)
: Received: 23 September 2016 / Approved: 26 September 2016 / Online: 26 September 2016 (09:25:39 CEST)
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Kakehashi, A.; Yoshida, M.; Tago, Y.; Ishii, N.; Gi, M.; Wanibuchi, H.; Okuno, T. Pueraria mirifica Exerts Estrogenic Effect and Promotes Mammary and Endometrial. Preprints2016, 2016080165 (doi: 10.20944/preprints201608.0165.v1).
Kakehashi, A.; Yoshida, M.; Tago, Y.; Ishii, N.; Gi, M.; Wanibuchi, H.; Okuno, T. Pueraria mirifica Exerts Estrogenic Effect and Promotes Mammary and Endometrial. Preprints 2016, 2016080165 (doi: 10.20944/preprints201608.0165.v1).
Pueraria mirifica (PM) is a plant which dried and powdered tuberous root is now widely used as a rejuvenating herb to promote youthfulness in both men and women. In this study, we investigated the modifying effects of PM at various doses on mammary and endometrial carcinogenesis in female Donryu rats. Firstly, PM administered to ovariectomized Donryu rats at doses of 0.03, 0.3 and 3% in phytoestrogen-low diet for 2 weeks induced the significant increases of uterus weight. Secondly, 4-week PM application to non-operated rats at a dose of 3% after the 7,12-dimethylbenz[a]anthracene (DMBA) initiation, resulted in significant elevation of cell proliferation in the mammary glands. In the third experiment, postpubertal administration of 0.3% (200 mg/kg b.w./day) PM to 5-week-old non-operated animals for 36 weeks following the initiation of mammary and endometrial carcinogenesis with DMBA and N-ethyl-N′-nitro-N-nitrosoguanidine (ENNG), respectively, caused significant increases of mammary adenocarcinoma incidence and multiplicity. A trend for increase of uterine adenocarcinomas and a significant increase of endometrial atypical hyperplasia multiplicity was observed at 0.3% PM. Furthermore, PM at doses of 0.3 and mostly 1% induced dilatation, hemorrhage and inflammation of the uterine wall. In conclusion, the postpubertal long-term PM administration to Donryu rats exerted estrogenic effect in the mammary gland and uterus, and at a dose of 200 mg/kg b.w./day promoted carcinogenesis initiated by DMBA and ENNG.
Pueraria mirifica; mammary gland; uterus; carcinogenesis; estrogenic activity; Donryu rat