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Driving Sustainable Asset Performance in Higher Education: The Roles of Governance, Digitalization, and Partnership Capabilities in Indonesia
Asmar Yulastri
,Ganefri
,Remon Lapisa
,Feri Ferdian
,Elfizon
,Marwan
,Arief Maulana
,Yudha Aditya Fiandra
Posted: 06 May 2026
Antibiotic Use and Care-Seeking Practices for Childhood Diarrhea and Respiratory Illnesses in the Community in Bangladesh
Sampa Dash
,Eva Sultana
,Md. Razibur Rahman
,Farina Naz
,Mohammad Ali
,ASG Faruque
,Subhra Chakraborty
Posted: 06 May 2026
Karamel’s Adventures: Building an AI-Powered Multilingual Storybook Generation Pipeline
Kahraman Kostas
Posted: 06 May 2026
Five-Meter Accuracy 3D Maps Illuminate Ancient Japan 1,800 Years Ago: Yamatai Queendom and First Emperor Jimmu
Masayuki Kanazawa
Posted: 06 May 2026
A qPCR Based Screening Platform for Exploratory Assessment of Phage Training Outcomes in Enterobacter cloacae and Stenotrophomonas maltophilia
Ghadeer Jdeed
,Vera Morozova
,Valeria Fedorets
,Tatiana Ushakova
,Lina Al Allaf
,Igor Babkin
,Nina Tikunovoa
Bacteriophages (phages) represent promising therapeutic agents. Their use in treatments is challenged by the rapid rise of resistant bacterial clones. To overcome this problem, phages can be trained in vitro to adapt them to the possible resistance that may arise. Here, we co-evolved phages with their hosts under different conditions and assessed the outcomes using qPCR. The co-evolution experiment yielded a panel of bacterial clones that were either adapted to a phage, a competing phage, or to a cocktail of both. The adaptation of a phage was done either in the continuous presence of an evolutionarily naïve host, or in a cocktail with a competing phage, or both conditions, or neither conditions. We assessed each obtained phage ability to infect evolved bacterial clones in the panel we created, and we used qPCR to enable high-throughput assessment. This allowed us to evaluate 500 phage-bacteria interactions. While all phages benefitted from the presence of evolutionary naïve hosts, the screening suggests that optimal training conditions are phage-specific, based on the four phages tested. For Enterobacter cloacae phages EC151 and EC152, the most extensive infectivity in our experiments was observed when a competing phage and/or an evolutionarily naïve host was included during adaptation. For Stenotrophomonas maltophilia phages StM171 and StenM174, the presence of an evolutionarily naïve hosts appeared beneficial in both replicates; co-adaptation with a competing phage led to a complete loss of StM171 infectivity in both experiments, but benefited StenM174. Phages passaged for 10 passages consistently infected a broader range of bacterial clones than those sampled after 5 passages. Sequencing of 8 phages obtained after adapting EC152 identified recurring mutations in a transcriptional regulator, and in some cases, in the baseplate and tail fiber genes.
Bacteriophages (phages) represent promising therapeutic agents. Their use in treatments is challenged by the rapid rise of resistant bacterial clones. To overcome this problem, phages can be trained in vitro to adapt them to the possible resistance that may arise. Here, we co-evolved phages with their hosts under different conditions and assessed the outcomes using qPCR. The co-evolution experiment yielded a panel of bacterial clones that were either adapted to a phage, a competing phage, or to a cocktail of both. The adaptation of a phage was done either in the continuous presence of an evolutionarily naïve host, or in a cocktail with a competing phage, or both conditions, or neither conditions. We assessed each obtained phage ability to infect evolved bacterial clones in the panel we created, and we used qPCR to enable high-throughput assessment. This allowed us to evaluate 500 phage-bacteria interactions. While all phages benefitted from the presence of evolutionary naïve hosts, the screening suggests that optimal training conditions are phage-specific, based on the four phages tested. For Enterobacter cloacae phages EC151 and EC152, the most extensive infectivity in our experiments was observed when a competing phage and/or an evolutionarily naïve host was included during adaptation. For Stenotrophomonas maltophilia phages StM171 and StenM174, the presence of an evolutionarily naïve hosts appeared beneficial in both replicates; co-adaptation with a competing phage led to a complete loss of StM171 infectivity in both experiments, but benefited StenM174. Phages passaged for 10 passages consistently infected a broader range of bacterial clones than those sampled after 5 passages. Sequencing of 8 phages obtained after adapting EC152 identified recurring mutations in a transcriptional regulator, and in some cases, in the baseplate and tail fiber genes.
Posted: 06 May 2026
Mineral Oxide-Mediated Transient Mucosal Signaling as a Framework for Enhanced Nutrient and Peptide Bioavailability: Hormesis, Tight Junction Physiology, and NRF2 Activation
Steven E. Warren
Posted: 06 May 2026
Looming Threat of Overwhelming Multiple Disease Outbreaks in Sudan: A Call for Urgent Action
Damilare M. Akintunde
,Omotolani O. Akintunde
Posted: 06 May 2026
V-Groove Channel Waveguides and Mach–Zehnder Interferometer in Hyperbolic van der Waals MoOCl₂
Olga Matveeva
,Kirill Voronin
,Maria Titova
,Sergey Chikalkin
,Andrey Vyshnevyy
,Aleksey Arsenin
,Valentyn Volkov
Posted: 06 May 2026
AA cGAN-Based Approach for SAR-to-Optical Image Translation Approach with Application to Cloud Removal
Ahmed Attia
,Peter Hofmann
Posted: 06 May 2026
The Anisotropic Etching Mechanism and Morphology Simulation of InP
Hui Zhang
,Lingfei Zhu
,Meng Zhu
Posted: 06 May 2026
Multi-Model Assessment and Experimental Validation of a Custom High-Camber Airfoil for Wind-Lens Technology Application
Ayalew Bekele Demie
,Venkata Ramayya Ancha
,Mulu Bayray Kahsay
Posted: 06 May 2026
Beyond Antibodies: How GFAP, NfL, Cytokines, Complement, miRNAs, Metabolomics, and the Microbiome Are Shaping Precision Medicine in NMOSD – A Narrative Review
Samira Ghobadzadeh
,Ali Salehnia Sammak
Posted: 06 May 2026
Theta and Alpha Oscillations Reflect Distinct Control and Stabilization Processes Across Working Memory
Adrián Ávila-Garibay
,Geisa B. Gallardo-Moreno
,Fabiola R. Gómez-Velázquez
,Steven Woltering
,Andrés A. González-Garrido
Posted: 06 May 2026
Hybrid Quantum-Classical Architectures in Medical Imaging: A Taxonomy-Based Survey of COVID-19 Models
Seyedeh Aram Salehi
,Hanieh Naderi
,Seyyed Amir Asghari
,Javad Chaharlang
,Yvon Savaria
Posted: 06 May 2026
A Regional Response to the Global Challenge of Single-Use Plastic Pollution: Regulatory Frameworks in IGAD Countries
Abdihakim Ahmed Mohamed
,Özlem Canbeldek Akın
Posted: 06 May 2026
A Unified Variational Principle for Reliable Machine Learning
Jose Manuel Velasco
,Beatriz Gonzalez
Posted: 06 May 2026
Integrated Phenotypic, Molecular, and Genomic Analysis of Antimicrobial Resistance in Yersinia pestis Isolates from Natural Plague Foci of Kazakhstan
Ziyat Abdel
,Zauresh Zhumadilova
,Raikhan Mussagalieva
,Aigul Abdirassilova
,Bolatbek Baitursyn
,Beck Abdeliyev
,Zhandos Dalibayev
,Dinmukhammed Otebay
,Nurbol Shaki
,Svetlana Issaeva
Posted: 06 May 2026
Mass Balance over Energy Balance: Why Direct Mass Accounting Offers a More Precise and Mechanistically Faithful Framework for Human Body Weight Regulation
Anssi H. Manninen
Posted: 06 May 2026
'A Large Quantity of Edibles, Too Much to Ruin Them All’. Dutch Colonial Language on Maroon Crop Diversity and Wild Plant Use
Tinde van Andel
Posted: 05 May 2026
Understanding the Impact of Different Nucleation Strategies on bis(2-Hydroxyethyl) Terephthalate Crystallization from a Glycolysis Reaction Mixture
Lukas Seppelfricke
,Henning Loos
,Leonard Sander
,Louisa-Marie Möller
,Kerstin Wohlgemuth
Posted: 05 May 2026
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