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Article
Social Sciences
Education

Asmar Yulastri

,

Ganefri

,

Remon Lapisa

,

Feri Ferdian

,

Elfizon

,

Marwan

,

Arief Maulana

,

Yudha Aditya Fiandra

Abstract: Effective asset management is critical for university sustainability, yet the mechanisms linking governance and digitalization to asset performance remain unclear, particularly in developing-country higher education. This study investigates how governance and digitalization influence sustainable asset performance in Indonesian public universities, focusing on the mediating role of partnership capabilities and the moderating roles of organizational readiness and environmental dynamism. Survey data were collected from 113 staff involved in asset management, governance, and partnerships across three Indonesian universities. Partial least squares structural equation modeling (PLS-SEM) was employed to test the hypothesized relationships. The results show that partnership capabilities fully mediate the effects of both governance and digitalization on sustainable asset performance. Neither governance nor digitalization exhibits significant direct effects. Organizational readiness moderates the governance-partnership capabilities relationship, while environmental dynamism does not significantly moderate the digitalization-partnership capabilities link. These findings extend dynamic capabilities theory to public university asset management and suggest that universities should prioritize building partnership capabilities, align digitalization investments with collaborative needs, and assess organizational readiness before implementing governance reforms.

Article
Public Health and Healthcare
Public Health and Health Services

Sampa Dash

,

Eva Sultana

,

Md. Razibur Rahman

,

Farina Naz

,

Mohammad Ali

,

ASG Faruque

,

Subhra Chakraborty

Abstract: Background: Antimicrobial resistance, driven by inappropriate and overuse of antibiotics, is a major public health threat. Diarrhea and respiratory illness are the leading causes of pediatric healthcare visits in low- and middle-income countries like Bangladesh. Despite clear WHO guidelines recommending limited use of antibiotics for these conditions, misuse is widespread. Methods: We interviewed caregivers of 3025 under-5 children via cellphones to assess common illnesses, associated care-seeking practices, and antibiotic use for diarrhea and respiratory illnesses experienced by their children in prior 14 days. Results: Among the participants, 116 (8.83%) reported diarrheal disease and 570 (18.84%) experienced respiratory illness during the preceding 2-week recall period. Among the children with diarrhea, 52.59% received antibiotics, and 73.77% obtained them over-the-counter from pharmacies. Among those with respiratory illness, 26.32% received antibiotics, with 58% procured them from local drugstores without a prescription from a registered physician. For diarrhea, azithromycin and metronidazole were the commonly used antibiotics; while for respiratory illness cefixime and azithromycin were frequently used. Notably, 68% of the diarrheal children either sought care from local drugstores, self-medicated, or did not receive any formal treatment. Conventional practice, long wait times at healthcare facilities, distance, and poverty were the main reasons for not seeking care from a registered healthcare provider. Conclusion: Understanding community-level antibiotic use and care-seeking behavior is essential to strengthen antibiotic stewardship and child health programs. Our findings highlight the urgent need for community awareness and enforcement of regulations restricting the over-the-counter sale of antibiotics to curb irrational and excessive antibiotic use.

Article
Computer Science and Mathematics
Artificial Intelligence and Machine Learning

Kahraman Kostas

Abstract: This paper presents a fully automated pipeline for converting monolingual, illustrated PDF storybooks into multilingual, AI-narrated interactive digital publications. The system was developed to disseminate 53 children's storybooks—originally produced in English by the Houston Education Attaché Office of the Republic of Türkiye and hosted at storiesofturkiye.com—across 34 target languages, covering the cultural, historical, and geographical heritage of Türkiye for young readers worldwide. The pipeline comprises four sequential stages: (1) structured PDF decomposition into text and image assets using PyMuPDF, (2) context-aware translation and editorial refinement via a locally hosted large language model (LLM) running under LM Studio, (3) multilingual text-to-speech (TTS) synthesis with optional zero-shot voice cloning using the Chatterbox model, and (4) automated generation of flip-book–style HTML5 web publications. The resulting system produces 15 languages with full audio-text output and an additional 19 languages with text-only output, reaching over 34 distinct linguistic communities through the diplomatic education network of Türkiye's overseas representations. We describe the architectural decisions, prompt engineering strategies, AI hallucination mitigation, and cross-lingual voice transfer challenges encountered, and we reflect on the broader implications of LLM-driven educational content localisation at scale.

Article
Arts and Humanities
Archaeology

Masayuki Kanazawa

Abstract: In this study, we employed the 5-meter Accuracy Digital Elevation Model (DEM) developed by the Geospatial Information Authority of Japan, to analyze the spatial distribution of Yayoi-period archaeological sites. Rather than relying on conventional regional cross-tabulations—such as prefecture-level classifications—this approach adopts a Geographic Information System (GIS)–based analysis that enables higher spatial precision as well as more intuitive and visually accessible interpretation. Through this methodology, we aim to reconstruct the geographical conditions of ancient Japan at the end of the Yayoi period, approximately 1,800 years ago, and to offer a new perspective on the long-standing debate concerning the location of Yamatai (Yamataikoku). The results of analyses using the 5m DEM substantially increase the likelihood that Yamatai was located in northern Kyushu. In addition, northern Kyushu exhibits highly distinctive patterns of land use that vary markedly by region. The areas surrounding present-day Asakura City and Ogori City appear to have been specialized primarily for military purposes. In contrast, the Yoshinogari site—one of the largest Yayoi-period settlements in Japan—shows a pronounced specialization in agriculture, particularly large-scale wet-rice cultivation. The area corresponding to modern Fukuoka City, meanwhile, functioned as a major urban center in which both military and agricultural functions were concentrated. Furthermore, the “Jimmu’s Eastern Expedition” undertaken by the first Emperor Jimmu cannot necessarily be dismissed as a purely legendary event; it likely reflects certain historical facts. By introducing a GIS-based approach that has been relatively underutilized in previous research, this study serves as a pilot project while simultaneously representing an ambitious attempt to expand the horizons of visualization in ancient Japanese historical studies.

Article
Biology and Life Sciences
Immunology and Microbiology

Ghadeer Jdeed

,

Vera Morozova

,

Valeria Fedorets

,

Tatiana Ushakova

,

Lina Al Allaf

,

Igor Babkin

,

Nina Tikunovoa

Abstract:

Bacteriophages (phages) represent promising therapeutic agents. Their use in treatments is challenged by the rapid rise of resistant bacterial clones. To overcome this problem, phages can be trained in vitro to adapt them to the possible resistance that may arise. Here, we co-evolved phages with their hosts under different conditions and assessed the outcomes using qPCR. The co-evolution experiment yielded a panel of bacterial clones that were either adapted to a phage, a competing phage, or to a cocktail of both. The adaptation of a phage was done either in the continuous presence of an evolutionarily naïve host, or in a cocktail with a competing phage, or both conditions, or neither conditions. We assessed each obtained phage ability to infect evolved bacterial clones in the panel we created, and we used qPCR to enable high-throughput assessment. This allowed us to evaluate 500 phage-bacteria interactions. While all phages benefitted from the presence of evolutionary naïve hosts, the screening suggests that optimal training conditions are phage-specific, based on the four phages tested. For Enterobacter cloacae phages EC151 and EC152, the most extensive infectivity in our experiments was observed when a competing phage and/or an evolutionarily naïve host was included during adaptation. For Stenotrophomonas maltophilia phages StM171 and StenM174, the presence of an evolutionarily naïve hosts appeared beneficial in both replicates; co-adaptation with a competing phage led to a complete loss of StM171 infectivity in both experiments, but benefited StenM174. Phages passaged for 10 passages consistently infected a broader range of bacterial clones than those sampled after 5 passages. Sequencing of 8 phages obtained after adapting EC152 identified recurring mutations in a transcriptional regulator, and in some cases, in the baseplate and tail fiber genes.

Review
Medicine and Pharmacology
Pharmacology and Toxicology

Steven E. Warren

Abstract: Background: Oral administration remains the preferred delivery route for supplements and bioactive compounds, yet the mucosal barrier restricts systemic exposure of most therapeutic agents to a small fraction of the administered dose. Enzymatic degradation, pH-dependent instability, and limited paracellular transport collectively constrain bioavailability, particularly for hydrophilic nutrients, peptides, and mineral ions. Framework: This paper proposes a three-mechanism framework to explain how a class of surface-active mineral oxide delivery systems may enhance mucosal absorption through established biological pathways. The framework is grounded entirely in peer-reviewed physiology and pharmacology independent of any proprietary formulation data. Mechanisms: The three proposed mechanisms are: (1) a low-dose, transient reactive oxygen species (ROS) pulse that reversibly modulates tight junction proteins, expanding paracellular permeability within a well-characterized physiological range; (2) surface-mediated delivery of mineral oxide species directly to mucosal epithelial cells, bypassing the portal circulation route that limits conventional oral mineral delivery; and (3) activation of the NRF2-KEAP1-ARE pathway in mucosal cells, upregulating endogenous antioxidant defenses through hormetic adaptation. These mechanisms are supported by the established biology of hormesis, tight junction physiology, mineral pharmacology, and redox signaling. Comparison to Existing Approaches: Liposomal encapsulation protects cargo from gastric degradation but achieves typical oral bioavailability of 1 to 15 percent for peptide cargo and faces instability challenges in the gastrointestinal environment. The mineral oxide delivery approach described here modifies the absorption environment itself rather than encapsulating cargo, representing a mechanistically distinct and potentially complementary strategy. Conclusion: This framework offers a biologically plausible, citation-supported basis for enhanced oral and sublingual delivery of nutrients and peptide compounds. Controlled clinical pharmacokinetic studies are needed to confirm these mechanisms and quantify the magnitude of bioavailability enhancement in human subjects.

Essay
Public Health and Healthcare
Public Health and Health Services

Damilare M. Akintunde

,

Omotolani O. Akintunde

Abstract: Sudan faces a looming threat of multiple overwhelming infectious disease outbreaks in 2026, driven primarily by conflict, displacement, and weakened health systems. Likely climate events, including floods, droughts, and heat waves, increase the likelihood and potential impact of outbreaks. Many epidemic-prone diseases have been identified by the WHO as health risks with high likelihood in the first third of 2026, including cholera, dengue fever, measles, acute enteric diseases (typhoid, rotavirus), acute respiratory tract infection, hepatitis E, meningococcal diseases, and Polio type 2; and classified as either very high risk or high risk based on the potential to cause morbidity/mortality. This is alongside other non-epidemic health crises such as malaria, malnutrition, and sexual and gender-based violence. There is an urgent need to strengthen key interventions, including the provision of safe water and sanitation and hygiene promotion for water-borne disease prevention and control, integrated vector surveillance and control for vector-borne diseases like dengue and malaria, and addressing low vaccination coverage through the re-establishment of routine immunizations and vaccination campaigns for diseases like diphtheria, pertussis, and meningitis. Strong community engagement (CE) remains central to preventing looming outbreak threats, alongside a commitment to coordination, funding, and implementation support for the Government of Sudan by UN agencies, including UNICEF and WHO, and humanitarian and development agencies, while ensuring localization. Collaborative efforts addressing vulnerabilities, healthcare preparedness, and community participation are required to limit the impact of the looming threat.

Communication
Physical Sciences
Optics and Photonics

Olga Matveeva

,

Kirill Voronin

,

Maria Titova

,

Sergey Chikalkin

,

Andrey Vyshnevyy

,

Aleksey Arsenin

,

Valentyn Volkov

Abstract: Miniaturization of photonic integrated circuits is a long-standing problem in optical engineering. Nowadays, the most promising material platform for integrated photonics are anisotropic van der Waals materials due to overcoming the light diffraction limit. Here, we numerically study v-groove channel waveguides formed in a 50-nm-thick slab of the in-plane hyperbolic in visible and near-infrared ranges van der Waals material MoOCl₂. At the telecom wavelength 1550 nm, a channel supports a guided mode with an effective index 1.0206 and a decay length of 13.7 µm. We also design a Mach–Zehnder–type interferometric layout with a maximum splitter angle of approximately 7° for demonstration of a possible practical application in a telecom range and in-plane angular channel modes propagation characteristics. We demonstrate that using MoOCl2 instead of gold leads to a tenfold reduction in the linear dimensions of the photonic integrated circuit. Therefore, we envision that by combining the extraordinary material properties of MoOCl2 with the v-shaped geometry of waveguides, one can make the integration density of photonic devices close to electronics.

Article
Environmental and Earth Sciences
Remote Sensing

Ahmed Attia

,

Peter Hofmann

Abstract: Cloud cover remains a persistent challenge in optical remote sensing, limiting the usability of optical satellite imagery for continuous Earth observation. Synthetic Aperture Radar (SAR) data, in contrast, provides cloud-penetrating, all-weather imaging but lacks the spectral richness and is less visually interpretable compared to optical observations. Bridging these complementary modalities, this study investigates SAR-to-optical image translation using the Pix2Pix conditional generative adversarial network (cGAN). While existing research predominantly focuses on reconstructing only the visible (RGB) or near-infrared bands, this work employs the winter subset of the SEN12-MS dataset to address the full spectral range. The objectives are threefold: (i) to validate SAR-to-optical translation across all 13 Sentinel-2 spectral bands; (ii) to assess the reliability and reconstructability of each individual band; and (iii) to evaluate the performance of the translation model for cloud removal. Experimental results show that the model effectively learns the SAR-to-optical mapping and achieves high reconstruction quality across all spectral bands, though bandwise analysis reveals that reconstruction accuracy varies with spectral characteristics. When applied to the SEN12-MS-CR dataset, the model successfully generates cloud-free optical imagery that closely matches reference data, achieving performance competitive with state-of-the-art models such as DiffCR. Overall, the findings confirm the viability of SAR-to-optical translation for producing spectrally consistent, cloud-free optical imagery, thus enhancing the temporal continuity of Earth observation data. Two ablation studies further analyze the impact of different loss functions and the exclusion of 60 m bands.

Article
Engineering
Mechanical Engineering

Hui Zhang

,

Lingfei Zhu

,

Meng Zhu

Abstract: As a third-generation semiconductor material, indium phosphide (InP) exhibits complex anisotropic etching characteristics, showing significantly varying etching morphologies under different temperature, concentration, and surfactant conditions. This complexity poses challenges in controlling the etching evolution process and predicting its three-dimensional structures. To address the simulation of InP etching structures and surface morphology, this study first establishes an atomic model of the InP etching system and analyzes how different atomic structures influence crystal plane etching rates. Subsequently, based on the microscopic activation energy theory, we propose an atomic removal determination function (InP-RPF) for InP etching substrates, numerically elucidating the relationship between macroscopic crystal plane etching rates and microscopic atomic removal probabilities. Furthermore, we develop an evolutionary Monte Carlo etching system model (InP-EMC), employing evolutionary algorithms to continuously optimize the energy parameters in the InP-RPF function, thereby adjusting the removal probabilities of various atomic types on the substrate and validating the simulated etching rates. Experimental comparisons demonstrate that the InP-EMC model accurately constrains atomic removal probabilities using limited crystal plane etching rate data, achieving simulation accuracy exceeding 90% for full-crystal-plane etching rates, mask etching structures, and surface morphology characteristics.

Article
Engineering
Energy and Fuel Technology

Ayalew Bekele Demie

,

Venkata Ramayya Ancha

,

Mulu Bayray Kahsay

Abstract: Diffusers in diffuser-augmented wind turbines (DAWTs) require high-camber airfoils operating at low Reynolds numbers (Re) and its laminar separation bubbles significantly complicate aerodynamic predictions. This study provides an experimental and numerical data for a custom-designed airfoil tested at Re = 68k–159k and angles of attack α = 0°–17.5°. Lift, drag, and pressure coefficient (Cp) distributions were measured experimentally. The XFOIL, the fully turbulent 3D k-ω SST, and the γ-Re_θ transition RANS models were validated against the experimental data using multiple quantitative metrics. The γ-Re_θ model demonstrated superior performance, achieving lift Maximum Absolute Percent Error of 1.6–3.4%, near-zero bias, and coefficient of determination > 0.99. It accurately captured the laminar separation bubble pressure plateau at mid-chord, with mean gross-averaged Cp percent errors of 8.1% and 2.1% for upper and lower surfaces, respectively. In contrast, the k-ω SST model overpredicted lift by up to +9.8% at Re = 68k and underpredicted drag by up to 66%. XFOIL showed poor reliability in transitional flow regimes. Sensitivity analyses confirmed the robustness of the γ-Re_θ model across the tested Re and α ranges. The generated experimental dataset, combined with the validated transition-sensitive RANS approach, provides a strong foundation for low-Re airfoil and DAWT diffuser design. Future work should extend experimental measurement below Re = 5x104 and above 2x105, including post-stall conditions and system level designing.

Review
Biology and Life Sciences
Immunology and Microbiology

Samira Ghobadzadeh

,

Ali Salehnia Sammak

Abstract: Neuromyelitis optica spectrum disorder (NMOSD) is a severe autoimmune astrocytopathy of the central nervous system, characterized by recurrent optic neuritis and longitudinally extensive transverse myelitis. Due to overlapping features with multiple sclerosis (MS), misdiagnosis remains common, yet treatment strategies differ fundamentally between these conditions. This narrative review synthesises recent advances in diagnostic and prognostic biomarkers for NMOSD, focusing on serological, cellular, molecular, and microbiological indicators.A targeted literature search was conducted in PubMed, Scopus and Web of Science (January 2015 – June 2025) using keywords related to NMOSD and biomarkers. Of over 500 initially identified articles, 72 key references were selected based on scientific relevance, methodological quality and clinical applicability, consistent with a narrative review approach.Established biomarkers – aquaporin‑4 immunoglobulin G (AQP4‑IgG) and myelin oligodendrocyte glycoprotein antibodies (MOG‑IgG) – remain central to diagnosis and phenotypic classification. Emerging markers, including glial fibrillary acidic protein (GFAP), neurofilament light chain (NfL), cytokines (interleukin‑6, CXCL13, CXCL10), complement components (C3, C4, sC5b‑9), microRNAs, metabolomics profiles, gut microbiome signatures and viral serology (Epstein–Barr virus, human herpesvirus 6), show promise in differentiating NMOSD from MS and MOGAD, predicting relapses, and guiding therapy. Integration of antibody assays with markers of astrocytic and axonal injury, immune activation and microbiological signatures could improve diagnostic accuracy and enable personalised treatment. However, clinical application remains limited by assay variability, lack of standardised cut-offs and small study populations. Future progress requires validated multimarker panels and harmonised measurement platforms across diverse populations.

Article
Biology and Life Sciences
Neuroscience and Neurology

Adrián Ávila-Garibay

,

Geisa B. Gallardo-Moreno

,

Fabiola R. Gómez-Velázquez

,

Steven Woltering

,

Andrés A. González-Garrido

Abstract: Background/Objectives: The oscillatory dynamics underlying stage-specific processing in working memory (WM) remain incompletely characterized, particularly under varying memory loads. We examined load-dependent modulation of theta (4-7 Hz), lower alpha (8-10 Hz), and upper alpha (11-13 Hz) absolute power during encoding, maintenance, and retrieval using quantitative EEG in a modified Sternberg task that temporally dissociates these stages. Methods: Forty-five healthy young adults performed trials with memory sets of three, five, or six uppercase consonants followed by a lowercase probe. EEG data were analyzed using cluster-based permutation testing, and brain-behavior relationships were assessed using regression models. Results: Fronto-central theta power increased with memory load and was significantly higher during retrieval than during encoding or maintenance. Greater theta power during retrieval predicted faster reaction times in the three-letter condition. Alpha oscillations showed robust stage effects. Lower alpha power was higher during maintenance than retrieval across loads and exhibited a load effect during maintenance (three > six letters) in occipital regions. Upper alpha power was consistently maximal during maintenance across all loads, involving bilateral fronto-central, parietal, and occipital regions. Critically, under moderate load (five letters), higher upper alpha power predicted a greater probability of correct responses across task stages. Conclusion: These findings demonstrate a functional dissociation between oscillatory bands across temporally separated WM stages: theta activity was retrieval-dominant and associated with response speed, whereas alpha, particularly upper alpha, was maintenance-dominant and supported accuracy under increased mnemonic demand.

Review
Computer Science and Mathematics
Artificial Intelligence and Machine Learning

Seyedeh Aram Salehi

,

Hanieh Naderi

,

Seyyed Amir Asghari

,

Javad Chaharlang

,

Yvon Savaria

Abstract: This paper surveys the emerging field of hybrid quantum–classical (HQC) architectures for medical image analysis, with a particular focus on COVID-19 classification as a rep-resentative application. Despite extensive progress in quantum machine learning, there is still a lack of structured taxonomy-driven analysis that systematically characterizes how quantum components are integrated within hybrid imaging pipelines. This study pro-vides a structured review of representative HQC models, categorizing existing approaches based on the functional role of quantum modules, including front-end processing, feature extraction, and classification layers. A comparative analysis is conducted to examine how these architectural design choices relate to reported performance across different ex-perimental settings. The review shows that hybrid models can achieve strong binary classification performance under simulation conditions, particularly when quantum cir-cuits are used as feature extractors. However, performance in multi-class scenarios remains inconsistent and highly sensitive to encoding strategies, circuit design, and optimization settings. In addition, most existing studies rely on simulator-based evaluations, limited or single-source datasets, and non-standardized benchmarking protocols, which restrict reproducibility and generalization. Real-hardware validation remains scarce, and no consistent evidence of task-level quantum advantage over optimized classical baselines has been demonstrated. Overall, current HQC-based approaches should be considered exploratory rather than clinically validated solutions. Future progress requires stand-ardized benchmarking, multi-institutional validation, hardware-aware evaluation, and the development of interpretable hybrid architectures that respect NISQ-era constraints.

Article
Arts and Humanities
Humanities

Abdihakim Ahmed Mohamed

,

Özlem Canbeldek Akın

Abstract: The issue of single-use plastic (SUP) waste is a recent sustainability challenge in developing and fragile regions with varying capacities for waste management, enforcement, and regional governance. This paper examines the regulation of SUPs in the Intergovernmental Authority on Development (IGAD) member states as an example of regional environmental governance in contexts of weak institutions. The paper provides a structured qualitative legal analysis of formally enacted legislative and policy measures. It assesses the extent to which national (and some subnational) legal responses comply with key principles of international environmental law, including prevention, the polluter-pays principle, and cooperation. The findings show that the legal responses to SUPs in IGAD are developing, but differ in scope, legal form, consistency, and effectiveness. Some countries employ direct bans, while others regulate plastics through their general environmental and waste management legislation. Prevention measures are prominent, but responsibility management measures (such as extended producer responsibility) are in their infancy, with Kenya, and to a lesser extent Uganda, showing more integrated systems. The paper links the regulation of SUPs to sustainable consumption and production, climate change, marine protection, effective institutions and partnerships, and informs discussions about SDGs 12, 13, 14, 16, and 17. The paper concludes that the most pragmatic way forward for IGAD is progressive regional harmonization, with enhanced common standards, monitoring, producer responsibility, and transboundary cooperation, rather than immediate legal convergence.

Article
Computer Science and Mathematics
Computational Mathematics

Jose Manuel Velasco

,

Beatriz Gonzalez

Abstract: Modern machine learning systems can achieve remarkable predictive performance. Nevertheless, in several fields, this is not enough to produce acceptable solutions as we need formal guarantees of robustness, fairness, and interpretability. Most existing approaches treat these properties separately or introduce them through external constraints, which makes their interaction difficult to analyze. In this work, we develop a unified variational perspective that incorporates these requirements directly into the learning objective. Concretely, we model learning as the minimization of a composite functional that combines predictive risk, regularization, and additional terms that capture robustness, fairness, and interpretability. This viewpoint allows us to study these properties within a single mathematical framework. Under standard assumptions, we prove the existence of minimizers and show that the resulting solutions are Pareto-optimal for the associated multi-objective problem. We illustrate the framework using examples based on adversarial and distributional robustness, statistical fairness criteria, and a notion of interpretability. The analysis points out the trade-offs that inevitably arise. We also examine statistical aspects of the proposed objective and show that classical generalization guarantees can still be obtained under appropriate conditions. The resulting framework provides a flexible basis for designing reliable learning systems.

Article
Biology and Life Sciences
Immunology and Microbiology

Ziyat Abdel

,

Zauresh Zhumadilova

,

Raikhan Mussagalieva

,

Aigul Abdirassilova

,

Bolatbek Baitursyn

,

Beck Abdeliyev

,

Zhandos Dalibayev

,

Dinmukhammed Otebay

,

Nurbol Shaki

,

Svetlana Issaeva

Abstract: Plague remains a globally significant zoonotic infection maintained in natural foci, with ongoing epizootic activity and periodic human cases reported in different regions of the world. Continuous monitoring of antimicrobial susceptibility of Yersinia pestis is essential due to the potential emergence and spread of resistant strains. A total of 75 Yersinia pestis isolates, including clinical and epizootic strains obtained from plague outbreaks in Kazakhstan, were analyzed. Antimicrobial susceptibility was evaluated using standard phenotypic methods, and molecular screening for resistance determinants was performed by real-time PCR. Genome-level analysis based on whole-genome sequencing (WGS) data from the NCBI BioProject PRJNA1249055 was conducted to assess the presence of acquired antimicrobial resistance genes and chromosomal mutations associated with resistance. All isolates demonstrated high susceptibility to clinically relevant antibiotics. No resistance genes were detected by molecular screening. Genome-based analysis confirmed the absence of acquired antimicrobial resistance determinants, resistance-associated mutations in key loci (rpsL, gyrA, parC), and plasmid-mediated resistance mechanisms. Minor lineage-associated variation in phoP was identified in a limited number of isolates and was not associated with antimicrobial resistance. These findings indicate a stable antimicrobial susceptibility profile of Yersinia pestis in Kazakhstan and confirm the absence of emerging resistance despite long-term circulation in natural plague foci. The results highlight the importance of integrated surveillance and support the continued effectiveness of current therapeutic strategies for plague.

Brief Report
Medicine and Pharmacology
Endocrinology and Metabolism

Anssi H. Manninen

Abstract: The energy balance model (EBM) and its operational form, calories-in-calories-out (CICO), have dominated obesity research for nearly a century. While these frameworks have delivered valuable public-health insights, they rest on indirect mass-to-energy conversions and persistent misconceptions about thermodynamic principles. Here I demonstrate that a first-principles mass balance model (MBM) provides a conceptually simpler, mathematically consistent, and mechanistically superior alternative. By tracking macronutrient mass directly in grams – without intermediate energy conversions – the MBM aligns analysis with physiological reality and delivers 40–65% lower propagated uncertainty than conventional energy-balance approaches. I clarify that calories cannot be eaten or oxidized, that E = mc² is irrelevant to human metabolism, and that the First Law of Thermodynamics concerns only energy, not mass. I further show that both the carbohydrate-insulin model and the recently proposed protein partitioning model remain anchored in the same energy-accounting framework the MBM transcends. Extending the analysis to practical domains, I demonstrate why the body senses mass restriction, not calorie restriction; why exercise induces weight loss only when it produces a net negative mass balance; how the MBM explains adaptive thermogenesis as an emergent property of mass-clearance down-regulation rather than an unexplained residual; and what mass-balance principles demand of pharmacotherapy and supplementation. Together, these arguments establish the MBM as a more parsimonious, mechanistically faithful, and clinically actionable paradigm for human body weight regulation.

Article
Biology and Life Sciences
Horticulture

Tinde van Andel

Abstract: The succesful escape from slavery between the late 17th and the mid 19thth century depended greatly on the runaway’s skills in adapting themselves to their natural environment. Although published works on the (oral) history of the Maroons hardly contain information on the gardens of the runaways, archival records of military expeditions aimed to destroy Maroon settlements report large provision fields, a variety of crops and ingenious uses of the surrounding forest. Vegetation types, cultivated crops and wild plants, indicated with archaic Dutch and Surinamese names, and written in a variety of spellings, are difficult to interpret for researchers studying archival records of this period. Still, these scholars have seldom collaborated with botanists to decipher such names. What crops did the Maroons grow in and around their hideouts? Which wild plant species were essential for their survival? Here we present a list of ultivated crops, wild useful plants and vegetation types that appear in colonial accounts on the violent destruction of the settlements of those who had sought freedom from the the harsh conditions of slavery. Although written by people who generaly despised Maroons, the accounts of the armed troops reflect the African agency in transforming plant use in the Americas. Their excellent agricultural skills and knowledge of the local flora helped the Maroons to survive and thrive in their hidden forest settlements. Although never mentioned by the reporters, these archival documents also reflect the plant knowledge of indigenous and enslaved militia members.

Article
Engineering
Chemical Engineering

Lukas Seppelfricke

,

Henning Loos

,

Leonard Sander

,

Louisa-Marie Möller

,

Kerstin Wohlgemuth

Abstract: The recycling of polyethylene terephthalate (PET) is gaining increasing importance, as it enables the conversion of plastic waste into valuable raw materials and contributes to a circular economy. Recent research has primarily focused on optimizing the depolymerization step of PET glycolysis, while downstream processes often overlooking the at least equally critical downstream steps in recovering the monomer bis(2-hydroxyethyl) terephthalate (BHET). The implementation of a water‑free PET glycolysis process eliminates challenges related to internal solvent and homogeneous catalyst recycling that commonly occur in conventional processes. This study therefore focuses on BHET crystallization and filtration as key downstream unit operations. Two nucleation strategies, gassing and seeding, were investigated and compared with experiments without a nucleation strategy. The aim was to achieve reproducible process control during crystallization and to obtain crystals with good filterability, which is essential for efficient washing and high product purity. Experiments without a nucleation strategy showed poor reproducibility. In contrast, gassing and seeding improved crystallization control, particularly regarding nucleation temperature and relative crystallization yield. However, these strategies also resulted in significantly prolonged filtration times due to differences in filter cake properties. The anisotropic crystals exhibited a broad particle size distribution with a high fraction of fine particles, leading to small and heterogeneous pores in the filter cake. Limited crystal growth was identified as the main cause of the unfavorable filtration behavior.

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