Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

β‐Carotene Impacts the Liver microRNA Profile in a Sex‐Specific Manner in Mouse Offspring of Western Diet‐Fed Mothers: Results from Microarray Analysis by Direct Hybridization

Diana Marisol Abrego-Guandique
ORCID logo ,
Sebastià Galmés
ORCID logo ,
Roberto Cannataro
,
Maria Cristina Caroleo
,
Joan Ribot
ORCID logo ,
M. Luisa Bonet
* ORCID logo ,
Erika Cione
ORCID logo
Version 1 : Received: 24 April 2024 / Approved: 25 April 2024 / Online: 25 April 2024 (16:06:08 CEST)

How to cite: Abrego-Guandique, D.M.; Galmés, S.; Cannataro, R.; Caroleo, M.C.; Ribot, J.; Bonet, M.L.; Cione, E. β‐Carotene Impacts the Liver microRNA Profile in a Sex‐Specific Manner in Mouse Offspring of Western Diet‐Fed Mothers: Results from Microarray Analysis by Direct Hybridization. Preprints 2024, 2024041692. https://doi.org/10.20944/preprints202404.1692.v1 Abrego-Guandique, D.M.; Galmés, S.; Cannataro, R.; Caroleo, M.C.; Ribot, J.; Bonet, M.L.; Cione, E. β‐Carotene Impacts the Liver microRNA Profile in a Sex‐Specific Manner in Mouse Offspring of Western Diet‐Fed Mothers: Results from Microarray Analysis by Direct Hybridization. Preprints 2024, 2024041692. https://doi.org/10.20944/preprints202404.1692.v1

Abstract

Maternal unbalanced diets cause adverse metabolic programming and affect the offspring's liver microRNA profile. The liver is a site of β-carotene (BC) metabolism and a target of BC action. We studied the interaction of maternal Western diet (WD) and early-life BC supplementation on the epigenetic remodeling of offspring’s liver microRNAs. Mouse offspring of WD-fed mothers were given daily a placebo (controls) or BC during suckling. The liver miRNAome was analyzed in recently weaned animals by microarray hybridization. BC impacted the liver miRNAome differently in male and female offspring, with no overlap in differentially expressed (DE) miRNAs between sexes and more impact in females. Bioinformatic analysis of DE miRNA predicted target genes revealed enrichment in biological processes/pathways related to metabolic processes, regulation of developmental growth and circadian rhythm, liver homeostasis and metabolism, insulin resistance, and neurodegeneration, among others, with differences between sexes. Fifty five percent of the overlapping target genes in both sexes identified were targeted by DE miRNAs changed in opposite directions in males and females. The results identify sex-dependent responses of the liver miRNA expression profile to BC supplementation during suckling and may sustain further investigations regarding the long-term impact of early postnatal life BC supplementation on top of an unbalanced maternal diet.

Keywords

Carotenoids; β-carotene; Liver; microRNAs; Western Diet; Lactation; Weaning; early-life programming

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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