Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Exogenous Cytokinin 4-PU-30 Modulates the Response of Wheat and Einkorn Seedlings to UV-B Radiation

Elisaveta Kirova
ORCID logo ,
Irina Moskova
,
Vasilissa Manova
,
Yana Koycheva
,
Zoia Tsekova
,
Denitsa Borisova
,
Hristo Nikolov
ORCID logo ,
Ventzeslav Dimitrov
,
Iskren Sergiev
ORCID logo ,
Konstantina Kocheva
* ORCID logo
Version 1 : Received: 23 April 2024 / Approved: 23 April 2024 / Online: 24 April 2024 (08:58:18 CEST)

How to cite: Kirova, E.; Moskova, I.; Manova, V.; Koycheva, Y.; Tsekova, Z.; Borisova, D.; Nikolov, H.; Dimitrov, V.; Sergiev, I.; Kocheva, K. Exogenous Cytokinin 4-PU-30 Modulates the Response of Wheat and Einkorn Seedlings to UV-B Radiation. Preprints 2024, 2024041554. https://doi.org/10.20944/preprints202404.1554.v1 Kirova, E.; Moskova, I.; Manova, V.; Koycheva, Y.; Tsekova, Z.; Borisova, D.; Nikolov, H.; Dimitrov, V.; Sergiev, I.; Kocheva, K. Exogenous Cytokinin 4-PU-30 Modulates the Response of Wheat and Einkorn Seedlings to UV-B Radiation. Preprints 2024, 2024041554. https://doi.org/10.20944/preprints202404.1554.v1

Abstract

Abiotic stress is responsible for significant reduction in crop plant productivity worldwide. Ultraviolet (UV) radiation is a natural component of sunlight and a permanent environmental stimulus. This study investigated the distinct responses of young wheat and einkorn plants to excessive UV-B radiation (180 min at λmax 312 nm) following foliar pretreatment with 1 µM synthetic cytokinin 4PU-30. Results demonstrated that UV radiation significantly amplified hydrogen peroxide levels in both wheat and einkorn, with einkorn exhibiting more pronounced increase compared to wheat. This elevation indicated the induction of oxidative stress by UV radiation in the two genotypes. Intensified antioxidant enzyme activities and increased accumulation of typical stress markers and non-enzyme protectants were evidenced. Transcriptional activity of genes encoding key antioxidant enzymes was also investigated aiming to elucidate the genetic regulation of the antioxidant systems in both wheat and einkorn seedlings. Our results suggested a role for POX1 and POX7 genes in the UV-B tolerance of the two wheat species as well as a cytokinin stimulated UV-B stress response in einkorn mediated through upregulation of the tau subfamily gene GSTU6. Based on these findings it could be concluded that 4PU-30 had the potential of alleviating oxidative stress by attenuating the symptoms of superfluous UV-B illumination in the two examined plant species.

Keywords

antioxidant gene expression; flavonoids; oxidative stress; phenolic compounds; phenylurea-cytokinin; proline; remote sensing; ROS; spectral reflectance

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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