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Assessing the In Vitro Individual and Combined Effect of Arthrobotrys Oligospora and A. musiformis (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode Haemonchus contortus (Trichostrongylidae)
Version 1
: Received: 15 April 2024 / Approved: 17 April 2024 / Online: 17 April 2024 (11:38:01 CEST)
How to cite:
Colinas-Picazo, A.; Mendoza-de Gives, P.; Pérez-Anzúrez, G.; Gutiérrez-Medina, E.; Bautista-García, G.A.; Delgado-Núñez, E.J.; Olmedo-Juárez, A. Assessing the In Vitro Individual and Combined Effect of Arthrobotrys Oligospora and A. musiformis (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode Haemonchus contortus (Trichostrongylidae). Preprints2024, 2024041103. https://doi.org/10.20944/preprints202404.1103.v1
Colinas-Picazo, A.; Mendoza-de Gives, P.; Pérez-Anzúrez, G.; Gutiérrez-Medina, E.; Bautista-García, G.A.; Delgado-Núñez, E.J.; Olmedo-Juárez, A. Assessing the In Vitro Individual and Combined Effect of Arthrobotrys Oligospora and A. musiformis (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode Haemonchus contortus (Trichostrongylidae). Preprints 2024, 2024041103. https://doi.org/10.20944/preprints202404.1103.v1
Colinas-Picazo, A.; Mendoza-de Gives, P.; Pérez-Anzúrez, G.; Gutiérrez-Medina, E.; Bautista-García, G.A.; Delgado-Núñez, E.J.; Olmedo-Juárez, A. Assessing the In Vitro Individual and Combined Effect of Arthrobotrys Oligospora and A. musiformis (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode Haemonchus contortus (Trichostrongylidae). Preprints2024, 2024041103. https://doi.org/10.20944/preprints202404.1103.v1
APA Style
Colinas-Picazo, A., Mendoza-de Gives, P., Pérez-Anzúrez, G., Gutiérrez-Medina, E., Bautista-García, G.A., Delgado-Núñez, E.J., & Olmedo-Juárez, A. (2024). Assessing the In Vitro Individual and Combined Effect of Arthrobotrys Oligospora and <em>A. musiformis</em> (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode <em>Haemonchus contortus</em> (Trichostrongylidae). Preprints. https://doi.org/10.20944/preprints202404.1103.v1
Chicago/Turabian Style
Colinas-Picazo, A., Edgar Jesús Delgado-Núñez and Agustín Olmedo-Juárez. 2024 "Assessing the In Vitro Individual and Combined Effect of Arthrobotrys Oligospora and <em>A. musiformis</em> (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode <em>Haemonchus contortus</em> (Trichostrongylidae)" Preprints. https://doi.org/10.20944/preprints202404.1103.v1
Abstract
The individual and combined effects of extracellular products (ECP) from Arthrobotrys oligospora (Ao) and A. musiformis (Am) growth in liquid media against Haemonchus contortus L3 (HcL3) were assessed. The following methodological steps were established: isolation, morphological (MI) and molecular identification (Mol-I), assessment of nematocidal activity (NA) of fungal liq-uid culture filtrates (LCF) produced in two liquid media alone and in combination and finally, the myco-compound profile identification (MCP). The MI of both isolates suggested that the fungi corresponded to the species Ao and Am. This result was confirmed by PCR analysis followed by sequencing, alignment and obtaining coverage and similarity with respect to isolates reported in the NCBI database. Likewise, the highest Hc mortalities were 91.4% with individual LCF of Am and 86.2% with those of Ao at the highest concentration (100 mg/mL) in Czapek-Dox Broth. The combination of both LCF resulted in a similarly high larval mortality with no statistical differ-ences in relation to individual activity (P>0.05). The MCP showed the presence of alkaloids in both fungi. Coumarins, triterpenes and saponins were found only in Ao. Both fungi produced ECP with a high NA that could be identified and assessed in future studies as potential natural an-thelmintic compounds.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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