PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
The Preventive Potential of the Mycelial Submerged Culture of Ophiocordyceps sinensis against Neurodegenerative Diseases and Its Possible Active Compounds
Version 1
: Received: 15 April 2024 / Approved: 15 April 2024 / Online: 15 April 2024 (11:29:23 CEST)
How to cite:
Huang, H.; Shi, Y.; Vo, T.; Hsu, T.; Song, T. The Preventive Potential of the Mycelial Submerged Culture of Ophiocordyceps sinensis against Neurodegenerative Diseases and Its Possible Active Compounds. Preprints2024, 2024040961. https://doi.org/10.20944/preprints202404.0961.v1
Huang, H.; Shi, Y.; Vo, T.; Hsu, T.; Song, T. The Preventive Potential of the Mycelial Submerged Culture of Ophiocordyceps sinensis against Neurodegenerative Diseases and Its Possible Active Compounds. Preprints 2024, 2024040961. https://doi.org/10.20944/preprints202404.0961.v1
Huang, H.; Shi, Y.; Vo, T.; Hsu, T.; Song, T. The Preventive Potential of the Mycelial Submerged Culture of Ophiocordyceps sinensis against Neurodegenerative Diseases and Its Possible Active Compounds. Preprints2024, 2024040961. https://doi.org/10.20944/preprints202404.0961.v1
APA Style
Huang, H., Shi, Y., Vo, T., Hsu, T., & Song, T. (2024). The Preventive Potential of the Mycelial Submerged Culture of Ophiocordyceps sinensis against Neurodegenerative Diseases and Its Possible Active Compounds. Preprints. https://doi.org/10.20944/preprints202404.0961.v1
Chicago/Turabian Style
Huang, H., Tai-Hao Hsu and Tuzz-Ying Song. 2024 "The Preventive Potential of the Mycelial Submerged Culture of Ophiocordyceps sinensis against Neurodegenerative Diseases and Its Possible Active Compounds" Preprints. https://doi.org/10.20944/preprints202404.0961.v1
Abstract
The pharmacological effects of the fruiting body of Ophiocordyceps sinensis (Os) exerted antioxidant, anti-virus, and immunomodulatory, whereas the anti-inflammatory effects and active components of Os mycelial submerged culture (OMSC) are not known. This study aimed to investigate the active compounds in the fermented liquid (FLOS), hot water (WEOS), and 50-95% (EEOS-50, EEOS-95) ethanol extracts of OMSC and their anti-inflammatory effects and potential mechanisms in LPS-stimulated microglial BV2 cells. The results demonstrated that all of the OMSC extracts could inhibit NO production in BV2 cells. EEOS-95 exhibited the strongest inhibitory effects (41 % inhibitory ability at 500 µg/mL), and its ergosterol, GABA, total phenolic, and total flavonoid contents were significantly higher than those of the other extracts (18.60, 18.60, 2.28, and 2.14 mg/g, p < 0.05, respectively). EEOS-95 also has a strong inhibitory ability against IL-6, IL-1β and TNF-α with IC50 of 617, 277 and 507 µg/mL, respectively, which is higher than that of 1 mM melatonin. The anti-inflammatory mechanism of EEOS-95 seems to be associated with up-regulation of PPAR-γ/Nrf-2/HO-1 antioxidant-related expression and down-regulation of NF-κB/COX-2/iNOS pro-inflammatory expression signaling. Therefore, we hypothesized that EEOS-95 can prevent neurodegenerative diseases, but the effect on the application in vivo should be further investigated.
Medicine and Pharmacology, Complementary and Alternative Medicine
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
