Version 1
: Received: 28 March 2024 / Approved: 29 March 2024 / Online: 29 March 2024 (11:50:05 CET)
How to cite:
Jung, H.; Sokolova, V.; Lee, G.; Stevens, V.; Tan, D. Structural and Biochemical Characterization of the Nucleosome Containing Variant H3.3 and H2A.Z. Preprints2024, 2024031829. https://doi.org/10.20944/preprints202403.1829.v1
Jung, H.; Sokolova, V.; Lee, G.; Stevens, V.; Tan, D. Structural and Biochemical Characterization of the Nucleosome Containing Variant H3.3 and H2A.Z. Preprints 2024, 2024031829. https://doi.org/10.20944/preprints202403.1829.v1
Jung, H.; Sokolova, V.; Lee, G.; Stevens, V.; Tan, D. Structural and Biochemical Characterization of the Nucleosome Containing Variant H3.3 and H2A.Z. Preprints2024, 2024031829. https://doi.org/10.20944/preprints202403.1829.v1
APA Style
Jung, H., Sokolova, V., Lee, G., Stevens, V., & Tan, D. (2024). Structural and Biochemical Characterization of the Nucleosome Containing Variant H3.3 and H2A.Z. Preprints. https://doi.org/10.20944/preprints202403.1829.v1
Chicago/Turabian Style
Jung, H., Victoria Stevens and Dongyan Tan. 2024 "Structural and Biochemical Characterization of the Nucleosome Containing Variant H3.3 and H2A.Z" Preprints. https://doi.org/10.20944/preprints202403.1829.v1
Abstract
Variant H3.3, along with H2A.Z, is notably enriched at promoter regions and is commonly associated with transcriptional activation. However, the specific molecular mechanisms through which H3.3 influences chromatin dynamics at transcription start sites, and its role in gene regulation, remain elusive. Using a combination of biochemistry and cryo-electron microscopy (cryo-EM), we show that the inclusion of H3.3 alone does not induce discernible changes in nucleosome DNA dynamics. Conversely, the presence of both H3.3 and H2A.Z enhances DNA flexibility similarly to H2A.Z alone. Interestingly, our findings suggest that the presence of H3.3 in the H2A.Z nucleosome provides slightly increased protection to DNA near the superhelical location (SHL) -5.5/5.5. These results imply that while H2A.Z at active promoters promotes the formation of more accessible nucleosomes with increased DNA accessibility to facilitate transcription, the simultaneous presence of H3.3 offers an additional mechanism to fine-tune nucleosome accessibility and the chromatin environment.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
