Ruszova, E.; Vanek, D.; Stühmer, W.; Khaznadar, Z.; Subhashini, N. The Utilization of the SaLux19-Based Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid and Sensitive Identification of Minute Amounts of a Biological Specimen. Life2024, 14, 579.
Ruszova, E.; Vanek, D.; Stühmer, W.; Khaznadar, Z.; Subhashini, N. The Utilization of the SaLux19-Based Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid and Sensitive Identification of Minute Amounts of a Biological Specimen. Life 2024, 14, 579.
Ruszova, E.; Vanek, D.; Stühmer, W.; Khaznadar, Z.; Subhashini, N. The Utilization of the SaLux19-Based Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid and Sensitive Identification of Minute Amounts of a Biological Specimen. Life2024, 14, 579.
Ruszova, E.; Vanek, D.; Stühmer, W.; Khaznadar, Z.; Subhashini, N. The Utilization of the SaLux19-Based Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid and Sensitive Identification of Minute Amounts of a Biological Specimen. Life 2024, 14, 579.
Abstract
We investigated the development of a simple and sensitive assay for the identification of minute amounts of animal and human biological material. The suggested system uses isothermal amplification of DNA in a rapid assay format, which returns the results in as little as 40 minutes from sampling. The assay used for the proof of concept employs the novel handheld SaLux19 device (Max-Planck Innovation prototype, Germany). This well-established infield detection system could also be utilized for forensic scenarios. The results presented here demonstrated that the assay is sufficiently specific and sensitive and can detect the presence of Sus scrofa nucleic acids.
Keywords
Species identification; LAMP; Point-of-Care Testing; SaLux19; Sus scrofa (pig)
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
