Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Isolation and Identification of Salinity-Tolerant Rhizobia from Soybean and Phenotypic Analysis of Nodulation in Germplasm

Version 1 : Received: 2 February 2024 / Approved: 5 February 2024 / Online: 5 February 2024 (10:52:36 CET)

How to cite: Yu, T.; Song, Y.; Wu, X.; Lv, H.; Zhang, G.; Tang, W.; Zheng, Z.; Wang, X.; Gu, Y.; Zhou, X.; Li, J.; Chen, Q.; Xin, D. Isolation and Identification of Salinity-Tolerant Rhizobia from Soybean and Phenotypic Analysis of Nodulation in Germplasm. Preprints 2024, 2024020211. https://doi.org/10.20944/preprints202402.0211.v1 Yu, T.; Song, Y.; Wu, X.; Lv, H.; Zhang, G.; Tang, W.; Zheng, Z.; Wang, X.; Gu, Y.; Zhou, X.; Li, J.; Chen, Q.; Xin, D. Isolation and Identification of Salinity-Tolerant Rhizobia from Soybean and Phenotypic Analysis of Nodulation in Germplasm. Preprints 2024, 2024020211. https://doi.org/10.20944/preprints202402.0211.v1

Abstract

The soybean yield per unit area and soybean acreage are increased to boost overall soybean production as two effective solutions. There is a large area of saline soils in northeast China, where if soybeans could be grown with the help of isolated saline-tolerant rhizobia, the cultivation area of soybeans in China can be effectively expanded. Several rhizobia with different salt-, acid- and alkali-resistant characteristics were obtained in this research by isolating, identifying, and analyzing the nitrogen-fixing activities of indigenous rhizobia from different soybean planting soils, and further analyzed the host compliance between rhizobia and different soybean germplasm concerning 30 representative germplasm resources in the northeast region. Collectively, the results of the resistance study of HLNEAU2 isolated from the Shaanxi region are great references for guiding the application of rhizobia on soybeans.

Keywords

soybean; isolation of rhizobia; resistance identification; nitrogen fixation capacity; host compliance

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

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