Acosta-Cárdenas, J.; Jiménez-García, L.F.; Cruz-Gómez, S.D.J.; Mendoza-von der Borch, A.P.; Segura-Valdez, M.D.L. Microscopic Analysis of Nuclear Speckles in a Viviparous Reptile. Preprints2024, 2024011821. https://doi.org/10.20944/preprints202401.1821.v1
APA Style
Acosta-Cárdenas, J., Jiménez-García, L.F., Cruz-Gómez, S.D.J., Mendoza-von der Borch, A.P., & Segura-Valdez, M.D.L. (2024). Microscopic Analysis of Nuclear Speckles in a Viviparous Reptile. Preprints. https://doi.org/10.20944/preprints202401.1821.v1
Chicago/Turabian Style
Acosta-Cárdenas, J., Ana Paulina Mendoza-von der Borch and María de Lourdes Segura-Valdez. 2024 "Microscopic Analysis of Nuclear Speckles in a Viviparous Reptile" Preprints. https://doi.org/10.20944/preprints202401.1821.v1
Abstract
Nuclear speckles are compartments enriched in splicing factors present in the nucleoplasm of mammalian cells. Their morphology is linked to the transcriptional and splicing activities of the cell through a recruitment mechanism. Speckles have been studied mostly in mammalian culture cells, and few studies are devoted to speckles in tissue cells. In mammals, speckles are present in different tissues and their morphology also depends on the hormonal cycle in rats. In the present work, we explore whether a similar situation is also present in non-mammalian tissue cells dur-ing the reproductive cycle. We studied the speckled pattern in several tissues of a viviparous rep-tile, the lizard Sceloporous torquatus, during two different stages of reproduction. We used immu-nofluorescence staining against splicing factors in hepatocytes and oviduct epithelium cells and fluorescence and confocal microscopy. The distribution of splicing factors in the nucleoplasm of oviductal cells and hepatocytes coincides with the nuclear speckled pattern described in mam-mals. In addition, the morphology of speckles varies in oviduct cells at the two stages of the re-productive cycle analyzed, paralleling the phenomenon observed in the rat. The results show that the morphology of speckles in reptile cells depends upon the reproductive stage as it occurs in mammals.
Keywords
cell nucleus; nuclear speckles; reptile; splicing factors; SR proteins; ribonucleoproteins
Subject
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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