Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Physical Characterization of Crosslinked Gelatin Hydrogel as a Potential Biomaterial for Gingival Tissue Regeneration

Dimas Ilham Hutomo
,
Fathia Agzarine Deandra
,
Ketherin Ketherin
,
Yuniarti Soeroso
* ,
Endang Winiati Bachtiar
ORCID logo ,
Fatimah Maria Tadjoedin
ORCID logo ,
Adityo Widaryono
,
Natalina Haerani
,
Robert Lessang
,
Elena García – Gareta
ORCID logo
Version 1 : Received: 24 January 2024 / Approved: 24 January 2024 / Online: 24 January 2024 (10:38:58 CET)

How to cite: Hutomo, D.I.; Deandra, F.A.; Ketherin, K.; Soeroso, Y.; Bachtiar, E.W.; Tadjoedin, F.M.; Widaryono, A.; Haerani, N.; Lessang, R.; Gareta, E.G.–. Physical Characterization of Crosslinked Gelatin Hydrogel as a Potential Biomaterial for Gingival Tissue Regeneration. Preprints 2024, 2024011755. https://doi.org/10.20944/preprints202401.1755.v1 Hutomo, D.I.; Deandra, F.A.; Ketherin, K.; Soeroso, Y.; Bachtiar, E.W.; Tadjoedin, F.M.; Widaryono, A.; Haerani, N.; Lessang, R.; Gareta, E.G.–. Physical Characterization of Crosslinked Gelatin Hydrogel as a Potential Biomaterial for Gingival Tissue Regeneration. Preprints 2024, 2024011755. https://doi.org/10.20944/preprints202401.1755.v1

Abstract

The development of biomaterial as substitution to connective tissue graft for treating gingival recession is growing nowadays. Hydrogels received attention as potential scaffold to be used in gingival tissue engineering since their capability to mimic soft tissue characteristic as they can hold amount of water. Gelatin is a natural polymer that widely used in hydrogel preparation with good biological properties, but it has poor physical stability for the used as scaffold. Chemical crosslinking strategy using EDC/NHS was performed in this study to overcome its limitation. One uncrosslinked gelatin and two crosslinked gelatin hydrogels different concentration of EDC/NHS were prepared in this study. This study investigated the difference of physical characterization from the prepared gelatin hydrogels. Our findings suggest the crosslinking strategy using EDC/NHS increase physical properties of the gelatin hydrogels without interfering the chemical structure of the gelatin. The higher crosslinker concentration will result in better morphology and more resilient to biodegradation. As a result, the crosslinked gelatin hydrogel with gelatin:EDC:NHS mass ratio of 12:1:1 met the physical characteristic for future use as scaffold for gingival tissue regeneration.

Keywords

gelatin hydrogel; gingival tissue regeneration; physical characteristic

Subject

Medicine and Pharmacology, Dentistry and Oral Surgery

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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