Version 1
: Received: 15 January 2024 / Approved: 15 January 2024 / Online: 16 January 2024 (02:16:37 CET)
How to cite:
Osei-Owusu, H.; Rondevaldova, J.; Houdkova, M.; Kudera, T.; Needham, T.; Kokoska, L. Evaluation of In Vitro Synergistic Interactions of Tetracycline with Alkaloid-Related Compounds against Diarrheagenic Bacterial Pathogens. Preprints2024, 2024011122. https://doi.org/10.20944/preprints202401.1122.v1
Osei-Owusu, H.; Rondevaldova, J.; Houdkova, M.; Kudera, T.; Needham, T.; Kokoska, L. Evaluation of In Vitro Synergistic Interactions of Tetracycline with Alkaloid-Related Compounds against Diarrheagenic Bacterial Pathogens. Preprints 2024, 2024011122. https://doi.org/10.20944/preprints202401.1122.v1
Osei-Owusu, H.; Rondevaldova, J.; Houdkova, M.; Kudera, T.; Needham, T.; Kokoska, L. Evaluation of In Vitro Synergistic Interactions of Tetracycline with Alkaloid-Related Compounds against Diarrheagenic Bacterial Pathogens. Preprints2024, 2024011122. https://doi.org/10.20944/preprints202401.1122.v1
APA Style
Osei-Owusu, H., Rondevaldova, J., Houdkova, M., Kudera, T., Needham, T., & Kokoska, L. (2024). Evaluation of In Vitro Synergistic Interactions of Tetracycline with Alkaloid-Related Compounds against Diarrheagenic Bacterial Pathogens. Preprints. https://doi.org/10.20944/preprints202401.1122.v1
Chicago/Turabian Style
Osei-Owusu, H., Tersia Needham and Ladislav Kokoska. 2024 "Evaluation of In Vitro Synergistic Interactions of Tetracycline with Alkaloid-Related Compounds against Diarrheagenic Bacterial Pathogens" Preprints. https://doi.org/10.20944/preprints202401.1122.v1
Abstract
Diarrhea-related mortality remains an important public health concern, particularly in developing countries, and has become difficult to treat because of antibacterial resistance. The development of synergistic antimicrobial agents appears to be a promising alternative treatment against diar-rheal infections. In this study, the combined effect of tetracycline together with either nitroxoline, sanguinarine, or zinc pyrithione (representing various classes of alkaloid-related compounds) was evaluated in vitro against selected diarrheagenic bacteria (Enterococcus faecalis, Escherichia coli, Listeria monocytogenes, Shigella flexneri, Vibrio parahaemolyticus, and Yersinia enterocolitica). The chequerboard method in 96-well microtiter plates was used to determine the sum of the fractional inhibitory concentration indices (FICIs). Three independent experiments were performed per combination, each in triplicate. It was observed that the combinations of tetracycline with either nitroxoline, sanguinarine, or zinc pyrithione produced synergistic effects against most of the pathogenic bacteria tested, with FICI values ranging from 0.086 to 0.5. Tetracycline-nitroxoline combinations produced the greatest synergistic action against S. flexneri at a FICI value of 0.086. Combinations of the agents tested in this study can thus be used for the development of new anti-diarrheal medications. However, studies focusing on their in vivo anti-diarrheal activity and safety are required before any consideration for utilization in human medicine.
Medicine and Pharmacology, Pharmacology and Toxicology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
,
,
