Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

OsLRR-RLP2 Gene Regulates Immunity to Magnaporthe oryzae in Japonica Rice

Hyo-Jeong Kim
,
Jeong Woo Jang
,
Thuy Pham
,
Van N.T. Nguyen
,
Ji-Hyun Kim
,
Chan woo Park
,
Yun-Shil Gho
ORCID logo ,
Eui-Jung Kim
ORCID logo ,
Soon-Wook Kwon
ORCID logo ,
Jong-Seong Jeon
ORCID logo ,
Sun Tae Kim
,
Ki Hong Jung
* ORCID logo ,
Yu-Jin Kim
* ORCID logo
Version 1 : Received: 11 January 2024 / Approved: 11 January 2024 / Online: 12 January 2024 (02:39:21 CET)

A peer-reviewed article of this Preprint also exists.

Kim, H.-J.; Jang, J.W.; Pham, T.; Tuyet, V.; Kim, J.-H.; Park, C.W.; Gho, Y.-S.; Kim, E.-J.; Kwon, S.-W.; Jeon, J.-S.; Kim, S.T.; Jung, K.-H.; Kim, Y.-J. OsLRR-RLP2 Gene Regulates Immunity to Magnaporthe oryzae in Japonica Rice. Int. J. Mol. Sci. 2024, 25, 2216. Kim, H.-J.; Jang, J.W.; Pham, T.; Tuyet, V.; Kim, J.-H.; Park, C.W.; Gho, Y.-S.; Kim, E.-J.; Kwon, S.-W.; Jeon, J.-S.; Kim, S.T.; Jung, K.-H.; Kim, Y.-J. OsLRR-RLP2 Gene Regulates Immunity to Magnaporthe oryzae in Japonica Rice. Int. J. Mol. Sci. 2024, 25, 2216.

Abstract

Rice is an important cereal crop worldwide, the growth of which is affected by rice blast disease, caused by the fungal pathogen Magnaporthe oryzae. As climate change increases the diversity of pathogens, the disease-resistance genes (R genes) in plants must be identified. The major blast-resistance genes have been identified in indica rice varieties; therefore, japonica rice varieties with R genes now need to be identified. Because leucine-rich repeat (LRR) domain proteins possess R-gene properties, we used bioinformatics analysis to identify the rice candidate LRR domain receptor-like proteins (OsLRR-RLPs). OsLRR-RLP2, which contains six LRR domains, showed differences in the DNA sequence, containing 43 single-nucleotide polymorphisms (SNPs) in indica and japonica subpopulations. The results of M. oryzae inoculation analysis indicated that indica varieties with partial deletion of OsLRR-RLP2 showed susceptibility, whereas japonica varieties with intact OsLRR-RLP2 showed resistance. oslrr-rlp2 mutant generated using clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) showed increased pathogen susceptibility, whereas plants overexpressing this gene showed pathogen resistance. These results indicate that OsLRR-RLP2 confers resistance to rice, and OsLRR-RLP2 may be useful for breeding resistant cultivars.

Keywords

LRR-RLP; Magnaporthe oryzae; rice; japonica resistance

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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