Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Oncostatin M Suppresses Bone Morphogenetic Protein-4-Induced Osteoprotegerin Synthesis in Osteoblasts: Attenuation of p70 S6 Kinase

Tomoyuki Hioki
ORCID logo ,
Junko Tachi
,
Rie Matsushima-Nishiwaki
,
Hiroki Iida
,
Osamu Kozawa
,
Haruhiko Tokuda
*
Version 1 : Received: 31 December 2023 / Approved: 2 January 2024 / Online: 2 January 2024 (09:38:17 CET)

How to cite: Hioki, T.; Tachi, J.; Matsushima-Nishiwaki, R.; Iida, H.; Kozawa, O.; Tokuda, H. Oncostatin M Suppresses Bone Morphogenetic Protein-4-Induced Osteoprotegerin Synthesis in Osteoblasts: Attenuation of p70 S6 Kinase. Preprints 2024, 2024010053. https://doi.org/10.20944/preprints202401.0053.v1 Hioki, T.; Tachi, J.; Matsushima-Nishiwaki, R.; Iida, H.; Kozawa, O.; Tokuda, H. Oncostatin M Suppresses Bone Morphogenetic Protein-4-Induced Osteoprotegerin Synthesis in Osteoblasts: Attenuation of p70 S6 Kinase. Preprints 2024, 2024010053. https://doi.org/10.20944/preprints202401.0053.v1

Abstract

Oncostatin M (OSM), an inflammatory cytokine belonging to interleukin-6 superfamily, is recognized as an essential factor secreted by osteal macrophages to orchestrate bone remodelling. Osteoprotegerin (OPG) produced by osteoblasts regulates osteoclastogenesis. We previously demonstrated that bone morphogenetic protein-4 (BMP-4) stimulates OPG synthesis in osteoblast-like MC3T3-E1 cells, and that SMAD1/5/8(9), p38 mitogen-activated protein kinase (MAPK), and p70 S6 kinase positively regulates the OPG synthesis. The present study is aimed to investigate the effect of OSM on BMP-4-stimulated OPG synthesis in osteoblasts. OSM suppressed the release and the mRNA expression of OPG upregulated by BMP-4 in MC3T3-E1 cells.  Neither the BMP-4-induced phosphorylation of SMAD1/5/9 nor that of p38 MAPK affected by OSM. On the other hand, the BMP-4-stimulated phosphorylation of p70 S6 kinase was considerably suppressed by OSM. These results strongly suggest that OSM suppresses the BMP-4-stimulated OPG synthesis via inhibition of the p70 S6 kinase-mediated pathway in osteoblasts.

Keywords

oncostatin M; bone morphogenetic protein-4; osteoprotegerin; p70 S6 kinase; osteoblast

Subject

Biology and Life Sciences, Endocrinology and Metabolism

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.