Version 1
: Received: 27 December 2023 / Approved: 28 December 2023 / Online: 28 December 2023 (15:13:16 CET)
How to cite:
Hernández-Marín, D. A.; Castro-Rios, R.; Chavez-Montes, A.; Salas-Báez, K. D.; Chávez-Gómez, R. I.; Muñoz-Ortega, M. H.; Sánchez-García, E. Trypanocidal Activity of Compounds Isolated From Parthenium incanum Kunth. Preprints2023, 2023122178. https://doi.org/10.20944/preprints202312.2178.v1
Hernández-Marín, D. A.; Castro-Rios, R.; Chavez-Montes, A.; Salas-Báez, K. D.; Chávez-Gómez, R. I.; Muñoz-Ortega, M. H.; Sánchez-García, E. Trypanocidal Activity of Compounds Isolated From Parthenium incanum Kunth. Preprints 2023, 2023122178. https://doi.org/10.20944/preprints202312.2178.v1
Hernández-Marín, D. A.; Castro-Rios, R.; Chavez-Montes, A.; Salas-Báez, K. D.; Chávez-Gómez, R. I.; Muñoz-Ortega, M. H.; Sánchez-García, E. Trypanocidal Activity of Compounds Isolated From Parthenium incanum Kunth. Preprints2023, 2023122178. https://doi.org/10.20944/preprints202312.2178.v1
APA Style
Hernández-Marín, D. A., Castro-Rios, R., Chavez-Montes, A., Salas-Báez, K. D., Chávez-Gómez, R. I., Muñoz-Ortega, M. H., & Sánchez-García, E. (2023). Trypanocidal Activity of Compounds Isolated From Parthenium incanum Kunth. Preprints. https://doi.org/10.20944/preprints202312.2178.v1
Chicago/Turabian Style
Hernández-Marín, D. A., Martín Humberto Muñoz-Ortega and Eduardo Sánchez-García. 2023 "Trypanocidal Activity of Compounds Isolated From Parthenium incanum Kunth" Preprints. https://doi.org/10.20944/preprints202312.2178.v1
Abstract
Parthenium incanum (Mariola) is used in Mexico to treat stomach and liver disorders. Reports have shown that the genus Parthenium contains biologically active metabolites. This work aimed to isolate and identify biologically active compounds with trypanocidal activity. The extracts were obtained through static maceration and extraction with successive reflux using different solvents. After that, they were fractionated with column chromatography, and the obtained fractions were compared by TLC; similar fractions were pooled and concentrated. A bioguided assay was performed with S. aureus ATCC 25923, using agar diffusion techniques and bioautography to determine preliminary biological activity. Active fractions were obtained and purified from a preparative TLC plate. The obtained compounds were identified as parthenin and coronopolin by TLC, FT-IR, and HPLC-MS. Finally, they were evaluated against the Ninoa strain of Trypanosoma cruzi, obtaining an IC50 of up to 45±2.5 ppm. It was concluded that P. incanum contains compounds with trypanocidal activity.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
*
