Version 1
: Received: 21 December 2023 / Approved: 21 December 2023 / Online: 21 December 2023 (06:45:27 CET)
Version 2
: Received: 8 February 2024 / Approved: 9 February 2024 / Online: 9 February 2024 (11:19:48 CET)
How to cite:
Ali, S.; Ali, U.; Jan, M. I. In-vivo hypolipidemic and in-silico HMG-CoA reductase inhibitory effects of 15-Oxoursolic acid from Rhododendron arboreum (Sm). Preprints2023, 2023121621. https://doi.org/10.20944/preprints202312.1621.v2
Ali, S.; Ali, U.; Jan, M. I. In-vivo hypolipidemic and in-silico HMG-CoA reductase inhibitory effects of 15-Oxoursolic acid from Rhododendron arboreum (Sm). Preprints 2023, 2023121621. https://doi.org/10.20944/preprints202312.1621.v2
Ali, S.; Ali, U.; Jan, M. I. In-vivo hypolipidemic and in-silico HMG-CoA reductase inhibitory effects of 15-Oxoursolic acid from Rhododendron arboreum (Sm). Preprints2023, 2023121621. https://doi.org/10.20944/preprints202312.1621.v2
APA Style
Ali, S., Ali, U., & Jan, M. I. (2024). In-vivo hypolipidemic and in-silico HMG-CoA reductase inhibitory effects of 15-Oxoursolic acid from Rhododendron arboreum (Sm). Preprints. https://doi.org/10.20944/preprints202312.1621.v2
Chicago/Turabian Style
Ali, S., Usman Ali and Muhammad Ishtiaq Jan. 2024 "In-vivo hypolipidemic and in-silico HMG-CoA reductase inhibitory effects of 15-Oxoursolic acid from Rhododendron arboreum (Sm)" Preprints. https://doi.org/10.20944/preprints202312.1621.v2
Abstract
Hyperlipidemia is an identified risk factor for metabolic syndrome, obesity and cardiovascular complications characterized by elevated levels of lipids in blood. The present study aimed to inhibit HMG-CoA reductase with 15-oxoursolic acid through in-vivo and in-silico assessments. HFD-induced hyperlipidemia in rats was developed with a marked increase in total cholesterol (58.34 ± 0.21 mg/dl), triglycerides (45.45 ± 0.39 mg/dl), LDL-c (66.12 ± 0.10 mg/dl), VLDL-c (19.34 ± 0.16 mg/dl), and a decrease in HDL-c (10.34 ± 0.18 mg/dl). The oral administration of 15-oxoursolic acid at a concentration of 30 mg/Kg b.w significantly reduced the serum concentration of total cholesterol (42.65 ± 0.54 mg/dl), triglycerides (32.33 ± 0.16 mg/dl), LDL-c (50.34 ± 0.15 mg/dl) and VLDL-c (13.15 ± 0.45 mg/dl) respectively and an increased in the serum HDL-c (18.56 ± 0.34 mg/dl). 15-oxo ursolic acid also caused a decrease in the coronary risk index and atherogenic risk index. In-silico studies of 15-oxoursolic acid with HMG-CoA reductase showed significant interaction capability with binding energy (-9.26805 Kcal/mol). It is concluded that 15-oxoursolic acid could significantly reduce the total cholesterol, triglycerides and LDL levels in HFD-induced hyperlipidemia rats which might lead to new medication with significant hypolipidemic potential.
Keywords
Hyperlipidemia, triterpenes, triglycerides, atherogenic index, coronary risk index
Subject
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
