Review
Version 1
Preserved in Portico This version is not peer-reviewed
Crafting Genetic Diversity: Unlocking the Potential of Protein Evolution
Version 1
: Received: 18 December 2023 / Approved: 19 December 2023 / Online: 19 December 2023 (10:45:59 CET)
A peer-reviewed article of this Preprint also exists.
Gali, V.K.; Tee, K.L.; Wong, T.S. Crafting Genetic Diversity: Unlocking the Potential of Protein Evolution. SynBio 2024, 2, 142-173. Gali, V.K.; Tee, K.L.; Wong, T.S. Crafting Genetic Diversity: Unlocking the Potential of Protein Evolution. SynBio 2024, 2, 142-173.
Abstract
Genetic diversity is the foundation of evolutionary resilience, adaptive potential, and the flourishing vitality of living organisms, serving as the cornerstone for robust ecosystems and the continuous evolution of life on Earth. The landscape of directed evolution, a powerful biotechnological tool inspired by natural evolutionary processes, has undergone a transformative shift propelled by innovative strategies for generating genetic diversity. This shift is fuelled by several factors, encompassing the utilization of advanced toolkits like CRISPR-Cas and base editors, enhanced comprehension of biological mechanisms, cost-effective custom oligo pool synthesis, and the seamless integration of artificial intelligence and automation. This comprehensive review looks into the myriad methodologies employed for constructing gene libraries, both in vitro and in vivo, categorized into three major classes: random mutagenesis, focused mutagenesis, and DNA recombination. The objectives of this review are threefold: firstly, to present a panoramic overview of recent advances in genetic diversity creation, secondly, to inspire novel ideas for further innovation in the genetic diversity generation, and thirdly, to provide a valuable resource for individuals entering the field of directed evolution.
Keywords
Protein engineering; directed evolution; genetic diversity; random mutagenesis; molecular cloning
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Comments (0)
We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.
Leave a public commentSend a private comment to the author(s)
* All users must log in before leaving a comment
