Cardioprotective Effect of Epiisopiloturine, an Imidazole Alkaloid from Pilocarpus microphyllus, in Rats Submitted to Cardiac Ischemia and Reperfusion

Pedro Ivo De Marqui Moraes; Fernando Sabia Tallo; Rildo Yamaguti Lima; Sandra Augusta Gordinho Pinto; Erisvaldo Amarante De Araújo; Rodrigo de Barros Freitas; Carolina Trabasso Ferraz De Oliveira; Giovanna Seto De Oliveira; João Vitor Gardelli Trindade; Alexia Nascimento Arias; Rafael Batman de Góes; Carlos Eduardo Braga Filho; Jand Venes Rolim Medeiros; Lucas Antonio Duarte Nicolau; Leiz Maria Costa Véras; Monica Noleto Miranda; Almir Gonçalves Wanderley; Afonso Caricati-Neto; Francisco Sandro Menezes-Rodrigues

doi:10.20944/preprints202311.1124.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 16 November 2023 / Approved: 17 November 2023 / Online: 17 November 2023 (05:22:27 CET)

Acute myocardial infarction (AMI), which is characterized by severe and fatal arrhythmias resultant from cardiac ischemia/reperfusion (CIR), is the leading cause of morbidity and mortality in the world. Considering that epiisopiloturin (EPI), an imidazole alkaloid found in the leaves of Pilocarpus microphyllus produded anti-inflammatory and antioxidant effects in an animal model of peritonitis, paw edema and peritonitis in mice, we decided to investigate the potential cardioprotective activity of EPI in animal model of CIR. Then, adult rats submitted to CIR pretreated with EPI 10 mg/kg (EPI10+CIR group) or 15 mg/kg (EPI15+CIR group) were compared to control animals submitted to CIR treated with saline solution 0.9% (CIR group). To evaluate the incidence of ventricular arrhythmias (VA), atrioventricular block (AVB) and lethality (LET) induced by CIR in these rats, the ECG analysis was performed. Serum levels of cardiac damage markers, total creatine kinase (CK) and CK-MB, were measured. The results showed that the AVB incidence was reduced from 80% to 30%, and LET incidence was reduced from 70% to 20%, compared to CIR group. Serum levels of LDH, total CK and, CK-MB was similar in all groups studied. These results indicate that the treatment with EPI (15 mg/kg, IV) before CIR was effective to produce cardioprotective effects. Thus, the use of EPI could be a promisse strategy to reduce the incidence of severe and fatal arrhythmias associated with AMI.

Cardiac Diseases; Cardiac Ischemia-Reperfusion; Myocardial Infarction; Pharmacological Cardioprotection; Epiisopiloloturine

Medicine and Pharmacology, Cardiac and Cardiovascular Systems

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Cardioprotective Effect of Epiisopiloturine, an Imidazole Alkaloid from Pilocarpus microphyllus, in Rats Submitted to Cardiac Ischemia and Reperfusion

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