Version 1
: Received: 13 November 2023 / Approved: 14 November 2023 / Online: 14 November 2023 (10:29:54 CET)
How to cite:
Edema, R.; Gordon, D.T. Genetic Determinants of Virulence of Two Clones of the Maize Streak Virus. Preprints2023, 2023110879. https://doi.org/10.20944/preprints202311.0879.v1
Edema, R.; Gordon, D.T. Genetic Determinants of Virulence of Two Clones of the Maize Streak Virus. Preprints 2023, 2023110879. https://doi.org/10.20944/preprints202311.0879.v1
Edema, R.; Gordon, D.T. Genetic Determinants of Virulence of Two Clones of the Maize Streak Virus. Preprints2023, 2023110879. https://doi.org/10.20944/preprints202311.0879.v1
APA Style
Edema, R., & Gordon, D.T. (2023). Genetic Determinants of Virulence of Two Clones of the Maize Streak Virus. Preprints. https://doi.org/10.20944/preprints202311.0879.v1
Chicago/Turabian Style
Edema, R. and Donald T Gordon. 2023 "Genetic Determinants of Virulence of Two Clones of the Maize Streak Virus" Preprints. https://doi.org/10.20944/preprints202311.0879.v1
Abstract
Using two infectious DNA clones originating from isolates obtained in Kenya, a genetic investigation of maize streak virus (MSV) pathogenicity was carried out. The susceptible Pioneer hybrid 3379 was used to investigate the virulence of kernels administered through vascular puncture inoculation. Mild [pMSV-KL (mild)] and severe [pMSV-Km(severe)] parental infectious MSV DNA clones were linked with considerable changes in the severity of chlorotic streaks and stunting of seedlings. Using restriction endonuclease fragments, chimeric clones were created from parental clones in order to investigate the genetic elements influencing these changes. Restrictions mapping was used to validate clone identities.
For genomic portions encoding the replication initiator (Rep and RepA), movement (MP), and coat (CP) protein ORFs as well as for those containing non-coding long (LIR) and short intergenic regions (SIR), putative virulence determinants were discovered. Recombinant clones with intermediate symptoms included LIRs together with the first 189 nt of the Rep/RepA ORFs from the pMSV-Km (Severe) and pMSV-KL (Mild) ORFs. Symptoms identical to those of parental clones were produced by complementary substitution of the Rep/RepA ORF. When compared to pMSV-Km (Severe), the recombinant clone having MP, CP, and SIR genome sequences from pMSV-KL(Mild) and LIR, Rep, and RepA genome sequences from pMSV-Km (Severe) displayed much more severe symptoms and accumulated greater concentrations. The reciprocal clone, in contrast, displayed much milder signs and virus titers than pMSV-KL(Mild). The level of leaf chlorosis and plant stunting were linked with viral accumulation.
These findings are consistent with the idea that MSV virulence is a polymorphic feature involving complementary sense genes (LIR and SIR) and the virion.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
