Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Epitope Mapping of an Anti-CD44v4 Monoclonal Antibody (C44Mab-108) using Enzyme-Linked Immunosorbent Assay

Hiroyuki Suzuki
* ORCID logo ,
Mayuki Tawara
,
Aoi Hirayama
,
Nohara Goto
,
Tomohiro Tanaka
,
Mika K Kaneko
,
Yukinari Kato
* ORCID logo
Version 1 : Received: 3 November 2023 / Approved: 3 November 2023 / Online: 3 November 2023 (06:38:38 CET)

A peer-reviewed article of this Preprint also exists.

Suzuki, H., Tawara, M., Hirayama, A., Goto, N., Tanaka, T., Kaneko, M. K., & Kato, Y. (2024). Epitope Mapping of an Anti-CD44v4 Monoclonal Antibody (C44Mab-108) Using Enzyme-Linked Immunosorbent Assay. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. Suzuki, H., Tawara, M., Hirayama, A., Goto, N., Tanaka, T., Kaneko, M. K., & Kato, Y. (2024). Epitope Mapping of an Anti-CD44v4 Monoclonal Antibody (C44Mab-108) Using Enzyme-Linked Immunosorbent Assay. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.

Abstract

CD44 is a type I transmembrane glycoprotein, and possesses various isoforms which are largely classified into CD44 standard and CD44 variant (CD44v) isoforms. Some variant-encoded regions play critical roles in tumor progression. However, the function of CD44 variant 4 (CD44v4)-encoded region has not been fully understood. Using peptide immunization, we developed an anti-CD44v4 mAb, C44Mab-108, which is useful for flow cytometry, western blotting, and immunohistochemistry. In this study, we determined the critical epitope of C44Mab-108 by enzyme-linked immunosorbent assay (ELISA). We used the alanine (or glycine)-substituted peptides of the CD44v4-encoded region (amino acids 271-290 of human CD44v3-10), and found that C44Mab-108 did not recognize the alanine-substituted peptides of D280A and W281A. Furthermore, these peptides could not inhibit the recognition of C44Mab-108 in flow cytometry and immunohistochemistry. The results indicate that the critical binding epitope of C44Mab-108 includes Asp280 and Trp281 of CD44v3-10.

Keywords

CD44; CD44 variant 4; monoclonal antibody; epitope; enzyme-linked immunosorbent assay

Subject

Medicine and Pharmacology, Oncology and Oncogenics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.