Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Content and Yield of L-DOPA and Bioactive Compounds of Broad Bean Plants: Antioxidant and Anti-Inflammatory Activity In Vitro

Paula Beatriz Fuentes-Herrera
ORCID logo ,
Braulio Edgar Herrera-Cabrera
ORCID logo ,
Alma Leticia Martínez-Ayala
,
Alejandro Zamilpa
ORCID logo ,
Adriana Delgado-Alvarado
* ORCID logo
Version 1 : Received: 4 October 2023 / Approved: 4 October 2023 / Online: 9 October 2023 (11:58:52 CEST)

A peer-reviewed article of this Preprint also exists.

Fuentes-Herrera, P.B.; Herrera-Cabrera, B.E.; Martínez-Ayala, A.L.; Zamilpa, A.; Delgado-Alvarado, A. Content and Yield of L-DOPA and Bioactive Compounds of Broad Bean Plants: Antioxidant and Anti-Inflammatory Activity In Vitro. Plants 2023, 12, 3918. Fuentes-Herrera, P.B.; Herrera-Cabrera, B.E.; Martínez-Ayala, A.L.; Zamilpa, A.; Delgado-Alvarado, A. Content and Yield of L-DOPA and Bioactive Compounds of Broad Bean Plants: Antioxidant and Anti-Inflammatory Activity In Vitro. Plants 2023, 12, 3918.

Abstract

The broad bean plant contains L-DOPA, a compound that is essential for patients with Parkin-son’s disease. However, little has been reported on other broad bean compounds that have bene-ficial effects on health. The objective was to evaluate plants of four Mexican broad bean varieties to determine the content and yield of total phenolic compounds (TPC), total flavonoids (TF), and L-DOPA, as well as to analyze the flavonoid profile and antioxidant (AA) and anti-inflammatory (AANTI) activity in vitro. Broad bean seeds were sown in the field and plants were harvested 20 days after emergence. The analyses were performed with visible UV spectrophotometry and HPLC. The variety José María produced the highest yield of TPC (9.30 g m-2), TF (8.08 g m-2) and L-DOPA (5.64 g m-2) per unit of area. The highest yields per plant were obtained with the Rojita variety: TPC (0.25 g plant-1), TF (0.21 g plant-1) and L-DOPA (0.17 g plant-1). This variety also had the highest antioxidant (CI50= 87.68 µg mL-¹) and anti-inflammatory (CI50= 74.40 mg mL-1) activi-ty, which was attributed to the L-DOPA compounds and to rutin and isoorientins, respectively. The flavonoid profile revealed the presence of rutin and isoorientins, which had not been previ-ously detected in the broad bean plant.

Keywords

Field; Parkinson’s disease; flavonoids; isoorientin; yield; rutin; Vicia faba L.

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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