Krüppel-like Factors Hypertrophy Modulation Following Isoproterenol-Induced Myocardial Damage

Michelle Giovanna Santoyo-Suarez; Juan Andres Garcia-Loredo; Jimena D. Mares-Montemayor; Lourdes Garza-Ocañas; Diego F. Benitez-Chao; Adolfo Soto-Dominguez; Ana E. Estrada Rodriguez; Oscar Rodriguez-Nuñez; Alberto Camacho-Morales; Patricio Adrián Zapata-Morín; Elsa N. Garza-Treviño; Juan Luis Delgado-Gallegos; Gerardo R. Padilla-Rivas; Jose Francisco Islas

doi:10.20944/preprints202308.2187.v2

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preprints.org > biology and life sciences > biochemistry and molecular biology > doi: 10.20944/preprints202308.2187.v2

Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

Krüppel-like Factors Hypertrophy Modulation Following Isoproterenol-Induced Myocardial Damage

Michelle Giovanna Santoyo-Suarez

Juan Andres Garcia-Loredo

Jimena D. Mares-Montemayor

Lourdes Garza-Ocañas

Diego F. Benitez-Chao

Adolfo Soto-Dominguez

Ana E. Estrada Rodriguez

Oscar Rodriguez-Nuñez

Alberto Camacho-Morales

Patricio Adrián Zapata-Morín

Elsa N. Garza-Treviño

Juan Luis Delgado-Gallegos

Gerardo R. Padilla-Rivas

Jose Francisco Islas

Version 1 : Received: 30 August 2023 / Approved: 31 August 2023 / Online: 31 August 2023 (12:01:14 CEST)
Version 2 : Received: 9 November 2023 / Approved: 10 November 2023 / Online: 13 November 2023 (10:02:18 CET)

How to cite: Santoyo-Suarez, M.G.; Garcia-Loredo, J.A.; Mares-Montemayor, J.D.; Garza-Ocañas, L.; Benitez-Chao, D.F.; Soto-Dominguez, A.; Estrada Rodriguez, A.E.; Rodriguez-Nuñez, O.; Camacho-Morales, A.; Zapata-Morín, P.A.; Garza-Treviño, E.N.; Delgado-Gallegos, J.L.; Padilla-Rivas, G.R.; Islas, J.F. Krüppel-like Factors Hypertrophy Modulation Following Isoproterenol-Induced Myocardial Damage. Preprints 2023, 2023082187. https://doi.org/10.20944/preprints202308.2187.v2 Santoyo-Suarez, M.G.; Garcia-Loredo, J.A.; Mares-Montemayor, J.D.; Garza-Ocañas, L.; Benitez-Chao, D.F.; Soto-Dominguez, A.; Estrada Rodriguez, A.E.; Rodriguez-Nuñez, O.; Camacho-Morales, A.; Zapata-Morín, P.A.; Garza-Treviño, E.N.; Delgado-Gallegos, J.L.; Padilla-Rivas, G.R.; Islas, J.F. Krüppel-like Factors Hypertrophy Modulation Following Isoproterenol-Induced Myocardial Damage. Preprints 2023, 2023082187. https://doi.org/10.20944/preprints202308.2187.v2

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MDPI and ACS Style

Santoyo-Suarez, M.G.; Garcia-Loredo, J.A.; Mares-Montemayor, J.D.; Garza-Ocañas, L.; Benitez-Chao, D.F.; Soto-Dominguez, A.; Estrada Rodriguez, A.E.; Rodriguez-Nuñez, O.; Camacho-Morales, A.; Zapata-Morín, P.A.; Garza-Treviño, E.N.; Delgado-Gallegos, J.L.; Padilla-Rivas, G.R.; Islas, J.F. Krüppel-like Factors Hypertrophy Modulation Following Isoproterenol-Induced Myocardial Damage. Preprints 2023, 2023082187. https://doi.org/10.20944/preprints202308.2187.v2

APA Style

Santoyo-Suarez, M.G., Garcia-Loredo, J.A., Mares-Montemayor, J.D., Garza-Ocañas, L., Benitez-Chao, D.F., Soto-Dominguez, A., Estrada Rodriguez, A.E., Rodriguez-Nuñez, O., Camacho-Morales, A., Zapata-Morín, P.A., Garza-Treviño, E.N., Delgado-Gallegos, J.L., Padilla-Rivas, G.R., & Islas, J.F. (2023). Krüppel-like Factors Hypertrophy Modulation Following Isoproterenol-Induced Myocardial Damage. Preprints. https://doi.org/10.20944/preprints202308.2187.v2

Chicago/Turabian Style

Santoyo-Suarez, M.G., Gerardo R. Padilla-Rivas and Jose Francisco Islas. 2023 "Krüppel-like Factors Hypertrophy Modulation Following Isoproterenol-Induced Myocardial Damage" Preprints. https://doi.org/10.20944/preprints202308.2187.v2

Abstract

Cardiac hypertrophy typically follows myocardial damage, a process known to involve members of the Krüppel-Like Factors (KLFs) which have pro-hypertrophic and anti-hypertrophic roles. Our study delved into the molecular mechanisms underlying KLFs-cardiac hypertrophy interplay post myocardial infraction. We induced myocardial dam-age in rats using isoproterenol. Total RNA was extracted from the left ventricle and Quantitative Real Time RT-PCR was conducted to assess the expression of KLFs, cardiac commitment genes, inflammatory markers, and certain conduction-related genes. We devel-oped a computational approach to construct a proteomic network centered on KLFs. Initial results revealed early expression (2-3 days post-induction) of KLF3, KLF4 and KLF6, followed by the subsequent expression of KLF11 and KLF15 (5-8 days after induction). In the maturation stage, KLF12 and KLF13 regulators were found upregulated. IL-6 was gradually found to be upregulated through. Interaction analysis revealed KLF3, KLF8 and KLF12 interacted with cardiac electrical functions. RT-PCR confirmed up-regulation in cardiac genes linked to electrical function and scar maturation. Our findings underscore the central role of KLFs during the modulation of the cardiac hypertrophic response. Dysregulation of KLF expression resulted in damaged myocardium, participating in the progression of abnormal hypertrophy, highlighting their potential as therapeutic targets for heart diseases.

Keywords

Krüppel-like factors; myocardial damage; cardiovascular diseases; hypertrophy.

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Received: 13 November 2023
Commenter: Jose Francisco Islas
Commenter's Conflict of Interests: Author

Comment: Expanded PCR, expanded on bioinformatics
added 1 author and need to change the name of another author

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