Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Circulating СD8+ T Cells Subsets in Primary Sjögren’s Syndrome

Igor Kudryavtsev
,
Stanislava Benevolenskaia
* ORCID logo ,
Maria Serebriakova
,
Irina Grigor’yeva
,
Evgeniy Kuvardin
,
Darina Zammoeva
,
Dmitri Motorin
,
Anna Budkova
,
Artem Rubinstein
ORCID logo ,
Alexey Golovkin
ORCID logo ,
Olga Kalinina
ORCID logo ,
Ekaterina Zaikova
ORCID logo ,
Sergey Vladimirovich Lapin
ORCID logo ,
Alexey Maslyanskiy
Version 1 : Received: 28 August 2023 / Approved: 29 August 2023 / Online: 30 August 2023 (08:15:07 CEST)

A peer-reviewed article of this Preprint also exists.

Kudryavtsev, I.; Benevolenskaya, S.; Serebriakova, M.; Grigor’yeva, I.; Kuvardin, E.; Rubinstein, A.; Golovkin, A.; Kalinina, O.; Zaikova, E.; Lapin, S.; Maslyanskiy, A. Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome. Biomedicines 2023, 11, 2778. Kudryavtsev, I.; Benevolenskaya, S.; Serebriakova, M.; Grigor’yeva, I.; Kuvardin, E.; Rubinstein, A.; Golovkin, A.; Kalinina, O.; Zaikova, E.; Lapin, S.; Maslyanskiy, A. Circulating CD8+ T Cell Subsets in Primary Sjögren’s Syndrome. Biomedicines 2023, 11, 2778.

Abstract

Currently, multiple studies have indicated that CD8+ T lymphocytes play a role in causing damage to the exocrine glands through acinar injury in Primary Sjogren’s syndrome (pSS). The aim of this research was to assess the imbalance of subsets within CD8+ cells in peripheral blood. We examined blood samples from 34 patients with pSS and 34 healthy individuals as controls. We used flow cytometry to enumerate CD8+ T cell maturation stages using as markers CD62L, CD28, CD27, CD4, CD8, CD3, CD45RA, and CD45. For immunophenotyping of ‘polarized’ CD8+ T cell subsets we used the following monoclonal antibodies:CXCR5, CCR6,CXCR3 and CCR4. The findings revealed that both the relative and absolute numbers of 'naïve' CD8+ T cells were higher in pSS patients compared to the healthy volunteers. Conversely, the proportions of effector memory CD8+ T cells were notably lower. Furthermore, our data suggested that among patients with pSS, the levels of cytotoxic Tc1 CD8+ T cells were reduced, while the frequencies of regulatory cytokine-producing Tc2 and Tc17 CD8+ T cells were significantly elevated. Simultaneously, the Tc1 cell subsets displayed a negative correlation with immunoglobulin G, Rheumatoid factor, the Schirmer test, and unstimulated saliva flow. On the other hand, the Tc2 cell subsets exhibited a positive correlation with these parameters. In summary, our study indicated that immune dysfunction within CD8+ T cells, including alterations in Tc1 cells, plays a significant role in the development of pSS.

Keywords

Sjögren's syndrome; T cell subsets; СD8+ T Cells

Subject

Medicine and Pharmacology, Internal Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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