Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Systematic Myostatin Expression Screening Platform for Identification and Evaluation of Myogenesis-Related Phytogenic in Economic Animal

Bor-Rung Ou
,
Ming-Hua Hsu
ORCID logo ,
Ling-Ya Haung
,
Chuan-Ju Lin
,
Li-Li Kuo
,
Yu-Ting Tsai
,
Yu-Chia Chang
,
Wen-Yuh Lin
ORCID logo ,
Tsung-Chien Huang
,
Yun-Chu Wu
,
Jan-Ying Yeh
* ,
Yu-Chuan Liang
*
Version 1 : Received: 28 August 2023 / Approved: 28 August 2023 / Online: 29 August 2023 (04:43:12 CEST)

A peer-reviewed article of this Preprint also exists.

Ou, B.-R.; Hsu, M.-H.; Haung, L.-Y.; Lin, C.-J.; Kuo, L.-L.; Tsai, Y.-T.; Chang, Y.-C.; Lin, W.-Y.; Huang, T.-C.; Wu, Y.-C.; Yeh, J.-Y.; Liang, Y.-C. Systematic Myostatin Expression Screening Platform for Identification and Evaluation of Myogenesis-Related Phytogenic in Pigs. Bioengineering 2023, 10, 1113. Ou, B.-R.; Hsu, M.-H.; Haung, L.-Y.; Lin, C.-J.; Kuo, L.-L.; Tsai, Y.-T.; Chang, Y.-C.; Lin, W.-Y.; Huang, T.-C.; Wu, Y.-C.; Yeh, J.-Y.; Liang, Y.-C. Systematic Myostatin Expression Screening Platform for Identification and Evaluation of Myogenesis-Related Phytogenic in Pigs. Bioengineering 2023, 10, 1113.

Abstract

Skeletal muscle is a specialized tissue consisting of nondividing and multinucleated muscle fibers. Skeletal muscle-specific myostatin (MSTN) is a secreted protein that acts as a negative regulator of muscle mass by inhibiting proliferation and differentiation of myoblasts. In this study, we established a screening platform to monitor myostatin promoter activity in rat L8 myoblasts. Extract of Glycyrrhiza uralensis (GUE), an oriental herbal medicine, was identified through this screening platform and, the active fractions of GUE were identified by process-scale liquid column chromatography system. For in vivo study, the GUE as a feed additive was investigated in growth-finishing pigs. The results showed that GUE significantly increased body weight, carcass weight and lean content in pigs. Microbiota analysis indicated that GUE did not affect the composition of gut microbiota in pigs. In summary, this established rodent myoblast screening platform was used to identify a myogenesis-related phytogenic, GUE, and further demonstrated that the active fractions inhibited MSTN expression. These finding suggest a novel application for GUE in growth performance enhancement through modulation of MSTN expression. Furthermore, this established screening platform has great potential for identification and evaluation of a wide variety of phytogenics involving in myogenesis.

Keywords

myogenesis; skeletal muscle; myostatin; Glycyrrhiza uralensis; feed additive

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.