Version 1
: Received: 23 August 2023 / Approved: 23 August 2023 / Online: 24 August 2023 (04:45:13 CEST)
How to cite:
Li, K.; Gong, X.; Jin, L.; Liu, M.; Hua, M. Analysis of 14 β-Agonists in Pork Using Automated Wooden Tip Based SPME Device and UPLC-MS/MS. Preprints2023, 2023081710. https://doi.org/10.20944/preprints202308.1710.v1
Li, K.; Gong, X.; Jin, L.; Liu, M.; Hua, M. Analysis of 14 β-Agonists in Pork Using Automated Wooden Tip Based SPME Device and UPLC-MS/MS. Preprints 2023, 2023081710. https://doi.org/10.20944/preprints202308.1710.v1
Li, K.; Gong, X.; Jin, L.; Liu, M.; Hua, M. Analysis of 14 β-Agonists in Pork Using Automated Wooden Tip Based SPME Device and UPLC-MS/MS. Preprints2023, 2023081710. https://doi.org/10.20944/preprints202308.1710.v1
APA Style
Li, K., Gong, X., Jin, L., Liu, M., & Hua, M. (2023). Analysis of 14 β-Agonists in Pork Using Automated Wooden Tip Based SPME Device and UPLC-MS/MS. Preprints. https://doi.org/10.20944/preprints202308.1710.v1
Chicago/Turabian Style
Li, K., Mengxiao Liu and Mengmeng Hua. 2023 "Analysis of 14 β-Agonists in Pork Using Automated Wooden Tip Based SPME Device and UPLC-MS/MS" Preprints. https://doi.org/10.20944/preprints202308.1710.v1
Abstract
This study presents an innovative analytical method for the detection and quantification of 14 β-agonists in pork using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method involves the use of a solid phase microextraction (SPME) probe, consisting of a wooden tip coated with polyacrylonitrile (PAN) and molecularly imprinted polymer (MIP). This probe has been integrated into an automated extraction device, designed in our laboratory to extract the target analytes prior to LC-MS/MS analysis. Compared to the traditional solid-phase extraction (SPE) procedure, the proposed approach significantly reduces the costs related to adsorbent ma-terial and solvent consumption, and shortens purification time, thereby reducing both the tech-nical workload and associated expenses. The methodology has been carefully validated in ac-cordance with the National Standard of the People's Republic of China (GB/T 22286-2008), evalu-ating key parameters including the limit of detection (LOD), limit of quantification (LOQ), matrix effects, linearity, and both intra-day and inter-day precision. The average recovery rates for all β-agonists were observed to lie between 71.6% and 82.2%, with relative standard deviations (RSDs) consistently below 15% for both types of precision. The LOD and LOQ values were found to range from 0.09 to 0.39 μg/kg and 0.27 to 0.99 μg/kg, respectively. In terms of accurate detection of positive samples, the developed method demonstrated a closer approximation to the true value of the sample compared to the National Standard method GB/T 31658.22-2022. Overall, the proposed method exhibits substantial promise for the routine assessment of β-agonists in pork and presents a practical alternative for compliance monitoring in regulatory settings.
Keywords
β-agonists; LC-MS/MS; automated SPME; pork
Subject
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
