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Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation
Belov, O.; Chigasova, A.; Pustovalova, M.; Osipov, A.; Eremin, P.; Vorobyeva, N.; Osipov, A.N. Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation. Curr. Issues Mol. Biol.2023, 45, 7352-7373.
Belov, O.; Chigasova, A.; Pustovalova, M.; Osipov, A.; Eremin, P.; Vorobyeva, N.; Osipov, A.N. Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation. Curr. Issues Mol. Biol. 2023, 45, 7352-7373.
Belov, O.; Chigasova, A.; Pustovalova, M.; Osipov, A.; Eremin, P.; Vorobyeva, N.; Osipov, A.N. Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation. Curr. Issues Mol. Biol.2023, 45, 7352-7373.
Belov, O.; Chigasova, A.; Pustovalova, M.; Osipov, A.; Eremin, P.; Vorobyeva, N.; Osipov, A.N. Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation. Curr. Issues Mol. Biol. 2023, 45, 7352-7373.
Abstract
Relative contribution of different repair pathways to the radiation-induced DNA damage responses remains a challenging issue of studying the radiation injury endpoints. Comparative manifestation of homologous recombination (HR) after application of different radiation doses greatly determines an overall effectiveness of recovery in dividing cell after irradiation, since HR is an error-free mechanism intended for repair of DNA double strand breaks (DSB) during S/G2 phases of cell cycle. In this article, we present an experimentally observed evidence of dose-dependent shift in relative contribution of HR in human fibroblasts after X-ray exposure at doses 20-1000 mGy, which is also supported by quantitative modeling of DNA DSB repair. Our findings indicate that the radiation dose increase leads to dose-dependent decrease in relative contribution of HR into the entire repair process.
Keywords
DNA double-strand breaks; ionizing radiation; DNA repair pathways; homologous recombination; mathematical modeling
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
