Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Sodium Butyrate Induces Mitophagy and Apoptosis of Bovine Skeletal Muscle Satellite Cells through the mTOR Signaling Pathway

Yanling Ding
† ,
Pengfei Wang
† ,
Chenglong Li
,
Yanfeng Zhang
,
Chaoyun Yang
ORCID logo ,
Xiaonan Zhou
,
Xiaowei Wang
,
Zonghua Su
,
Wenxuan Ming
,
Ling Zeng
,
Yuangang Shi
,
Cong-Jun Li
* ,
Xiaolong Kang
* ORCID logo
†
These authors have contributed equally to this work and share first authorship.
Version 1 : Received: 20 July 2023 / Approved: 21 July 2023 / Online: 21 July 2023 (05:18:58 CEST)

A peer-reviewed article of this Preprint also exists.

Ding, Y.; Wang, P.; Li, C.; Zhang, Y.; Yang, C.; Zhou, X.; Wang, X.; Su, Z.; Ming, W.; Zeng, L.; Shi, Y.; Li, C.-J.; Kang, X. Sodium Butyrate Induces Mitophagy and Apoptosis of Bovine Skeletal Muscle Satellite Cells through the Mammalian Target of Rapamycin Signaling Pathway. Int. J. Mol. Sci. 2023, 24, 13474. Ding, Y.; Wang, P.; Li, C.; Zhang, Y.; Yang, C.; Zhou, X.; Wang, X.; Su, Z.; Ming, W.; Zeng, L.; Shi, Y.; Li, C.-J.; Kang, X. Sodium Butyrate Induces Mitophagy and Apoptosis of Bovine Skeletal Muscle Satellite Cells through the Mammalian Target of Rapamycin Signaling Pathway. Int. J. Mol. Sci. 2023, 24, 13474.

Abstract

Sodium butyrate (NaB) is one of the short-chain fatty acids and is notably produced in large amounts from dietary fiber in the gut. Recent evidence suggests that NaB induces cell proliferation and apoptosis. The skeletal muscle is rich in plenty of mitochondrial. However, it is unclear how NaB acts on host muscle cells and whether it is involved in mitochondria-related functions in myocytes. The present study aimed to investigate the role of NaB treatment on the proliferation, apoptosis, and mitophagy of bovine skeletal muscle satellite cells (BSCs). The results showed that NaB inhibited the proliferation and promoted apoptosis of BSCs, and promoted mitophagy in a time and dose-dependent manner in BSCs. In addition, 1mM NaB increased the mitochondrial ROS level, decreased the mitochondrial membrane potential (MMP), increased the number of autophagic vesicles in mitochondria, and increased the mitochondrial DNA(mtDNA) and ATP level. The effects of the mTOR pathway on BSCs were investigated, and the results showed that 1mM NaB inhibited the mRNA and protein expression of mTOR and genes AKT1, FOXO1, and EIF4EBP1 in the mTOR signaling pathway, while the addition of PP242, an inhibitor of the mTOR signaling pathway, also inhibited mRNA and protein expression levels of mTOR, AKT1, FOXO1, and EIF4EBP1, and promoted mitophagy and apoptosis, which were consistent with the effect of NaB treatment. In conclusion, NaB might promote mitophagy and apoptosis in BSCs by inhibiting the mTOR signaling pathway. Our results would expand the knowledge of sodium butyrate on bovine skeletal muscle cell state and mitochondrial function.

Keywords

bovine skeletal muscle satellite cells; sodium butyrate; mitophagy; apoptosis; mTOR signaling pathway

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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